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| Description
| - We used suppression subtractive hybridization to search for genes more expressed in fetal hind limb muscles of Piétrain pigs compared to adult biceps femoris. The following genes were studied: FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS. By sequencing PCR-generated genomic fragments of a Piétrain and a Meishan pig, SNPs and indels were identified in all studied genes except for YWHAQ. Polymorphisms were tested by PCR-RFLP. Unrelated pigs of eight breeds (Large White, Landrace, Piétrain, Black Pied Prestice, Czech Meat Pig, Hampshire, Duroc and Meishan) were genotyped and allele frequencies are presented. Linkage mapping was performed in the Hohenheim Meishan x Piétrain (MxP) and/or Wild Boar x Meishan (WxM) three-generation pedigrees. RH mapping was performed using the porcine whole-genome 7000 rad IMpRH panel exept of CNN3 and POSTN.
- We used suppression subtractive hybridization to search for genes more expressed in fetal hind limb muscles of Piétrain pigs compared to adult biceps femoris. The following genes were studied: FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS. By sequencing PCR-generated genomic fragments of a Piétrain and a Meishan pig, SNPs and indels were identified in all studied genes except for YWHAQ. Polymorphisms were tested by PCR-RFLP. Unrelated pigs of eight breeds (Large White, Landrace, Piétrain, Black Pied Prestice, Czech Meat Pig, Hampshire, Duroc and Meishan) were genotyped and allele frequencies are presented. Linkage mapping was performed in the Hohenheim Meishan x Piétrain (MxP) and/or Wild Boar x Meishan (WxM) three-generation pedigrees. RH mapping was performed using the porcine whole-genome 7000 rad IMpRH panel exept of CNN3 and POSTN. (en)
- Použili jsme subtraktivní hybridizaci k výběru genů více exprimovaných ve svalovině plodu než dospělého jedince u prasat plemene pietrain. Byly studovány následující geny: FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 a GNAS. Byly identifikovány SNP a in/del polymorfizmy u většiny genů s vyjímkou YWHAQ. Polymorfizmy byly testovány PCR-RFLP a jsou prezentovány frekvence alel a genotypů. Bylo provedeno vyzbové a RH mapování. (cs)
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| Title
| - Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
- Mapování prasečích genů FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS, exprimovaných v kosterní svalovině plodu (cs)
- Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles (en)
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| skos:prefLabel
| - Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
- Mapování prasečích genů FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS, exprimovaných v kosterní svalovině plodu (cs)
- Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles (en)
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| skos:notation
| - RIV/62156489:43210/08:00114109!RIV09-GA0-43210___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA523/03/0858), P(GA523/06/1302)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/08:00114109
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - subtractive hybridization; polymorphism (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Horák, Pavel
- Knoll, Aleš
- Svobodová, Kateřina
- Bílek, Karel
- Bartenschlager, Heinz
- Geldermann, Hermann
- Stratil, Antonín
- Bechyňová, R.
- Peelman, Luck J.
- Van Poucke, Mario
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
of | - Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
- Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
- Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
- Mapping of the porcine FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 and GNAS genes, expressed in foetal skeletal muscles
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