| Attributes | Values |
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| rdf:type
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| Description
| - Dva polární benzo[c]fenanthridinové alkaloidy, chelerythrin (CHE) a dihydrochelerythrin (DHCHE), byly extrahovány při 35 °C a 10 MPa (15 MPa u reálných vzorků) z reálných a spajkovaných vzorků plasmy s přijatelnými návratnostmi (95.1 % and 81.0 %) pomocí superkritického oxidu uhličitého modifikovaného směsí (1:1, v/v) voda/methanol. Alkaloidy byly stanoveny HPLC/ESI-MS na koloně Zorbax SB-CN gradientovou elucí mobilní fázi methanol/50 mM mravenčan amonný. LOD byl 1.22 ng (3.50 pmol) pro CHE a 0.95 ng (2.72 pmol) pro DHCHE v 1 ml vzorku. Průměrná návratnost byla 100.1 % pro spajkovaný i reálný vzorek extraktů plasmy. Metodou byly extrahovány a analyzovány reálné vzorky plasmy potkanů. (cs)
- Two polar benzo[c]phenanthridine alkaloids, chelerythrine (CHE) and dihydrochelerythrine (DHCHE), were extracted at 35 °C and 10 MPa (15 MPa for real samples) from real and spiked plasma samples with acceptable recoveries (95.1 % and 81.0 %, respectively) using near-critical CO2 modified with aqueous (1:1, v/v) methanol. The alkaloids were quantified by a liquid chromatographic/electrospray mass spectrometric (LC/ESI-MS) method on a Zorbax SB-CN column (75 x 4.6 mm, 3.5 mm particle size) using methanol (organic phase) and 50 mM ammonium formiate (aqueous phase) as a mobile phase. A linear gradient 0 to 1 min - isocratic at 60% aqueous phase (v/v); from 1.0 to 7.0 min - 60% to 71% aqueous phase (v/v); from 7.0 to 18.0 min - 71% to 60% aqueous phase (v/v) was applied. The limit of detection was 1.22 ng (3.50 pmol) for CHE and 0.95 ng (2.72 pmol) for DHCHE per 1 ml of the sample. The linearity of the calibration curves was satisfactory as indicated by coefficients of determination 0.9979 and 0.9995 for C
- Two polar benzo[c]phenanthridine alkaloids, chelerythrine (CHE) and dihydrochelerythrine (DHCHE), were extracted at 35 °C and 10 MPa (15 MPa for real samples) from real and spiked plasma samples with acceptable recoveries (95.1 % and 81.0 %, respectively) using near-critical CO2 modified with aqueous (1:1, v/v) methanol. The alkaloids were quantified by a liquid chromatographic/electrospray mass spectrometric (LC/ESI-MS) method on a Zorbax SB-CN column (75 x 4.6 mm, 3.5 mm particle size) using methanol (organic phase) and 50 mM ammonium formiate (aqueous phase) as a mobile phase. A linear gradient 0 to 1 min - isocratic at 60% aqueous phase (v/v); from 1.0 to 7.0 min - 60% to 71% aqueous phase (v/v); from 7.0 to 18.0 min - 71% to 60% aqueous phase (v/v) was applied. The limit of detection was 1.22 ng (3.50 pmol) for CHE and 0.95 ng (2.72 pmol) for DHCHE per 1 ml of the sample. The linearity of the calibration curves was satisfactory as indicated by coefficients of determination 0.9979 and 0.9995 for C (en)
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| Title
| - High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples
- High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples (en)
- HPLC/ESI-MS stanovení sanguinarinu a chelerythrinu po extrakci superkritickým oxidem uhličitým zs spajkkovaných a reálných vzorků plasmy. (cs)
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| skos:prefLabel
| - High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples
- High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples (en)
- HPLC/ESI-MS stanovení sanguinarinu a chelerythrinu po extrakci superkritickým oxidem uhličitým zs spajkkovaných a reálných vzorků plasmy. (cs)
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| skos:notation
| - RIV/62156489:43210/07:00099035!RIV08-GA0-43210___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/07:00099035
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - HPLC; alkaloids; plasma samples (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kosina, Pavel
- Kubáň, Vlastimil
- Ulrichová, Jitka
- Klejdus, Bořivoj
- Šimánek, Vilím
- Lojková, Lea
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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