| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Deriváty platnatých a platičitých komplexů mají jako alkylační látky nezastupitelnou roli v léčbě nádorových onemocnění. Jako první byla do terapie zavedena cisplatina (1965), později karboplatina a oxaliplatina. Spektrum jejich protinádorového účinku je široké a zahrnuje nádory varlat, plic, prsu, prostaty a mnohé další. Protinádorový mechanismus účinku cisplatiny je dobře prozkoumán in vitro na nádorových buněčných liniích, nikdy však nebyl sledován vliv cisplatiny na rostlinné buňky. Cílem naší práce bylo ověření cytotoxického účinku cisplatiny na rostlinné buňky suspenzní buněčné kultury Nicotiana tabacum BY-2. Bylo zjišťováno ovlivnění životnosti buněk suspenzní kultury tabáku v průběhu kultivace s přídavkem cisplatiny v různých koncentracích v kultivačním médiu za použití metod fluorescenční mikroskopie. Dále byl stanoven mitotický index. Hodnocena byla rovněž morfologická stavba jádra (jadérko, heterochromatin a euchromatin) a průběh buněčného dělení se zaměřením na jednotlivé fáze mitózy. (en)
- Derivatives of platinous and platinic complex with strong alkylating effect have important role in treatment of malignant disorders. Cisplatin was introduced to the therapy of these disorders in 1965 followed by carboplatin and oxaliplatin. The spectrum of their cytostatic effects is wide and influences carcinomas of testicles, pulmonary, breast, prostate and of many others. The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied on plant cell lines. The main aim of our work was to study cytotoxic effect of cisplatin on plant cell suspension culture of Nicotiana tabacum BY-2. We observed changing of their viability with increasing cisplatin concentration in culture medium using fluorescent microscopy. Mitotic index was studied too. We also measured morphological structure of nuclei (nucleolus, heterochromatin and euchromatin) as well as mitosis and single phases of mitosis.
- Derivatives of platinous and platinic complex with strong alkylating effect have important role in treatment of malignant disorders. Cisplatin was introduced to the therapy of these disorders in 1965 followed by carboplatin and oxaliplatin. The spectrum of their cytostatic effects is wide and influences carcinomas of testicles, pulmonary, breast, prostate and of many others. The mechanism of cisplatin effect has been well examined in in vitro models of malignant cell lines, but has never been studied on plant cell lines. The main aim of our work was to study cytotoxic effect of cisplatin on plant cell suspension culture of Nicotiana tabacum BY-2. We observed changing of their viability with increasing cisplatin concentration in culture medium using fluorescent microscopy. Mitotic index was studied too. We also measured morphological structure of nuclei (nucleolus, heterochromatin and euchromatin) as well as mitosis and single phases of mitosis. (cs)
|
| Title
| - VLIV CISPLATINY NA BUNĚČNOU SUSPENZNÍ KULTURU TABÁKU NICOTIANA TABACUM BY-2
- VLIV CISPLATINY NA BUNĚČNOU SUSPENZNÍ KULTURU TABÁKU NICOTIANA TABACUM BY-2 (cs)
- An influence of cisplatin on tobacco cell culture Nicotiana tabacum BY-2 (en)
|
| skos:prefLabel
| - VLIV CISPLATINY NA BUNĚČNOU SUSPENZNÍ KULTURU TABÁKU NICOTIANA TABACUM BY-2
- VLIV CISPLATINY NA BUNĚČNOU SUSPENZNÍ KULTURU TABÁKU NICOTIANA TABACUM BY-2 (cs)
- An influence of cisplatin on tobacco cell culture Nicotiana tabacum BY-2 (en)
|
| skos:notation
| - RIV/62156489:43210/05:00086832!RIV06-GA0-43210___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/05:00086832
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Cell viability; Cisplatin; Nicotiana tabacum BY-2 (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...v/mistoKonaniAkce
| |
| http://linked.open...i/riv/mistoVydani
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Babula, Petr
- Havel, Ladislav
- Petrlová, Jitka
- Sladký, Zdeněk
- Kízek, René
|
| http://linked.open...vavai/riv/typAkce
| |
| http://linked.open.../riv/zahajeniAkce
| |
| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Mendelova zemědělská a lesnická univerzita v Brně
|
| https://schema.org/isbn
| |
| http://localhost/t...ganizacniJednotka
| |
| is http://linked.open...avai/riv/vysledek
of | |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)