About: Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Kinetics of in-gel digestion of proteins by modified and native trypsins was studied by MALDI TOF mass spectrometry using O-18-labeled peptides as internal standards. The effect of the temperature, enzyme concentration, digestion time, and surface area of gel pieces on the yield of digestion products was characterized. Based on the kinetic data, we developed a protocol that enabled the identification of gel-separated proteins with 30-min digestion time without compromising the peptide yield and the sensitivity compared to conventional protocols that typically rely upon overnight enzymatic cleavage. The accelerated digestion protocol was tested in identification of more than 120 proteins from budding and fission yeasts at the subpicomole level. Kinetics of in-gel digestion of proteins by modified and native trypsins was studied by MALDI TOF mass spectrometry using O-18-labeled peptides as internal standards. The effect of the temperature, enzyme concentration, digestion time, and surface area of gel pieces on the yield of digestion products was characterized. Based on the kinetic data, we developed a protocol that enabled the identification of gel-separated proteins with 30-min digestion time without compromising the peptide yield and the sensitivity compared to conventional protocols that typically rely upon overnight enzymatic cleavage. The accelerated digestion protocol was tested in identification of more than 120 proteins from budding and fission yeasts at the subpicomole level. (en)
Title	Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins (en)
skos:prefLabel	Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins (en)
skos:notation	RIV/61989592:15410/03:00001376!RIV/2004/MSM/154104/N
http://linked.open.../vavai/riv/strany	1300-1306
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 153100013)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Šebela, Marek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Pedagogická fakulta
http://linked.open...dnocenehoVysledku	607106
http://linked.open...ai/riv/idVysledku	RIV/61989592:15410/03:00001376
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	FLIGHT MASS-SPECTROMETRY, FUNCTIONAL PROTEOMICS, POLYACRYLAMIDE GELS, TRYPTIC DIGESTION, IDENTIFICATION, QUADRUPOLE, TOOL, COMPLEXES, PEPTIDES, TRYPSIN (en)
http://linked.open.../riv/klicoveSlovo	COMPLEXES TOOL IDENTIFICATION FUNCTIONAL PROTEOMICS POLYACRYLAMIDE GELS QUADRUPOLE TRYPSIN TRYPTIC DIGESTION PEPTIDES FLIGHT MASS-SPECTROMETRY
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[9A2D065F2A9E]
http://linked.open...i/riv/nazevZdroje	Analytical Chemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	75
http://linked.open...iv/tvurceVysledku	Šebela, Marek Havliš, Jan Shevchenko, Andrej Thomas, Henrik
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100013
issn	0003-2700
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	15410

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