About: A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeastSerbia: inference for pea domestication.     Goto   Sponge   NotDistinct   Permalink

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Description
  • The origin of the agriculture was one of key points in human history, and a central part of this was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. Domesticated in Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. We present the molecular analysis of ancient DNA extracted from carbonized pea seeds recovered from the 11th century B.C. deposits at Hissar, in southeast Serbia. To distinguish between cultivated and wild gathered pea, two samples were subjected to sequence analysis of fragments of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1,329 bp. This resulted in intermediate position of ancient pea between extant cultivated P. sativum and wild Pisum sativum subsp. elatius. The level of the detected mutations in the amplified DNA fragments proved, that genuine ancient DNA, was analysed. Based on combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of coloured flower and pigmented testa, similar to today's fodder pea (Pisum sativum subsp. sativum var. arvense), possibly of winter type. This is the first report of successful ancient DNA extraction and analysis from any legume species so far. The implications to pea domestication are discussed.
  • The origin of the agriculture was one of key points in human history, and a central part of this was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. Domesticated in Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. We present the molecular analysis of ancient DNA extracted from carbonized pea seeds recovered from the 11th century B.C. deposits at Hissar, in southeast Serbia. To distinguish between cultivated and wild gathered pea, two samples were subjected to sequence analysis of fragments of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1,329 bp. This resulted in intermediate position of ancient pea between extant cultivated P. sativum and wild Pisum sativum subsp. elatius. The level of the detected mutations in the amplified DNA fragments proved, that genuine ancient DNA, was analysed. Based on combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of coloured flower and pigmented testa, similar to today's fodder pea (Pisum sativum subsp. sativum var. arvense), possibly of winter type. This is the first report of successful ancient DNA extraction and analysis from any legume species so far. The implications to pea domestication are discussed. (en)
Title
  • A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeastSerbia: inference for pea domestication.
  • A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeastSerbia: inference for pea domestication. (en)
skos:prefLabel
  • A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeastSerbia: inference for pea domestication.
  • A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeastSerbia: inference for pea domestication. (en)
skos:notation
  • RIV/61989592:15310/14:33150453!RIV15-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S
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http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 591
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/14:33150453
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • pea; legumes; Early Iron Age; domestication; archaeogenetics; archaeobotany; ancient DNA (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [B679CB642A21]
http://linked.open...i/riv/nazevZdroje
  • Genetic Resources and Crop Evolution
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 61
http://linked.open...iv/tvurceVysledku
  • Smýkal, Petr
  • Čupina, Branko
  • Mikič, Aleksandar
  • Dordevic, Vuk
  • Jovanocič, Živko
  • Medovič, Aleksandar
  • Stanisavljevic, Nemanja
  • Zlatkovic, Bojan
http://linked.open...ain/vavai/riv/wos
  • 000345377800008
issn
  • 0925-9864
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s10722-014-0128-z
http://localhost/t...ganizacniJednotka
  • 15310
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