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| rdf:type
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| rdfs:seeAlso
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| Description
| - A simple carbon paste (CP) electrode, modified with novel maghemite (gama-Fe2O3) nanoparticles, called SAMNs (suface active maghemite nanoparticles) and characterized by a mean diameter of about 10 nm, has been developed. The electrode catalyzes the electro-reduction of hydrogen peroxide at low applied potentials (?0.1 V vs SCE). In order to improve the electrocatalytic properties of the modified electrode an ionic liquid, namely 1-butyl-3-methylimidazolium hexafluorophosphate (BMIM-PF6), was introduced. At ?0.1 V, the sensitivity of the SAMN-BMIM-PF6-CP electrode was 206.51 nA mýM?1 cm?2, with a detection limit (S/N=3) of 0.8 mýM, in the 0-1.5 mM H2O2 concentration range. Furthermore, glucose oxidase was immobilized on the surface of maghemite nanoparticles as a monomolecular layer, by a bridge constituted of rhodamine B isothiocyanate, leading to a fluorescent, magnetic drivable nanocatalyst, containing 10+-2 enzyme molecules per nanoparticle. The resulting enzyme electrode presents a linear calibration curve toward glucose in solution in the concentration range of 0-1.5 mM glucose, characterized by a sensitivity of 45.85 nA mýM?1 cm?2 and a detection limit (S/N=3) of 0.9 mýM. The storage stability of the system was evaluated and a half-life of 2 months was calculated, if the electrode is stored at 4 °C in buffer. The present work demonstrates the feasibility of these surface active maghemite nanoparticles as efficient hydrogen peroxide electro-catalyst, which can be easily coupled to hydrogen peroxide producing enzymes in order to develop oxidase based reagentless biosensor devices.
- A simple carbon paste (CP) electrode, modified with novel maghemite (gama-Fe2O3) nanoparticles, called SAMNs (suface active maghemite nanoparticles) and characterized by a mean diameter of about 10 nm, has been developed. The electrode catalyzes the electro-reduction of hydrogen peroxide at low applied potentials (?0.1 V vs SCE). In order to improve the electrocatalytic properties of the modified electrode an ionic liquid, namely 1-butyl-3-methylimidazolium hexafluorophosphate (BMIM-PF6), was introduced. At ?0.1 V, the sensitivity of the SAMN-BMIM-PF6-CP electrode was 206.51 nA mýM?1 cm?2, with a detection limit (S/N=3) of 0.8 mýM, in the 0-1.5 mM H2O2 concentration range. Furthermore, glucose oxidase was immobilized on the surface of maghemite nanoparticles as a monomolecular layer, by a bridge constituted of rhodamine B isothiocyanate, leading to a fluorescent, magnetic drivable nanocatalyst, containing 10+-2 enzyme molecules per nanoparticle. The resulting enzyme electrode presents a linear calibration curve toward glucose in solution in the concentration range of 0-1.5 mM glucose, characterized by a sensitivity of 45.85 nA mýM?1 cm?2 and a detection limit (S/N=3) of 0.9 mýM. The storage stability of the system was evaluated and a half-life of 2 months was calculated, if the electrode is stored at 4 °C in buffer. The present work demonstrates the feasibility of these surface active maghemite nanoparticles as efficient hydrogen peroxide electro-catalyst, which can be easily coupled to hydrogen peroxide producing enzymes in order to develop oxidase based reagentless biosensor devices. (en)
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| Title
| - A glucose biosensor based on surface active maghemite nanoparticles
- A glucose biosensor based on surface active maghemite nanoparticles (en)
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| skos:prefLabel
| - A glucose biosensor based on surface active maghemite nanoparticles
- A glucose biosensor based on surface active maghemite nanoparticles (en)
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| skos:notation
| - RIV/61989592:15310/13:33145795!RIV14-AV0-15310___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ED2.1.00/03.0058), P(KAN115600801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/13:33145795
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Glucose biosensor; Hydrogen peroxide detection; Superparamagnetic nanoparticles; Nanomaterial electrocatalysis; gama-Fe2O3; Maghemite nanoparticles (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Biosensors and Bioelectronics
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Zbořil, Radek
- Baratella, Davide
- Magro, Massimiliano
- Salviulo, Gabriella
- Vianello, Fabio
- Sinigaglia, Giulietta
|
| http://linked.open...ain/vavai/riv/wos
| |
| issn
| |
| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1016/j.bios.2013.01.043
|
| http://localhost/t...ganizacniJednotka
| |
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