About: Update on Methods and Techniques to Study Endocytosis in Plants

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The growing interest in the investigation of endocytosis, vesicular transport routes, and corresponding regulatory mechanisms resulted in the exploitation of cell biological, genetic, biochemical, and proteomic approaches. Methods and techniques such as site-directed and T-DNA insertional mutagenesis, RNAi, classical inhibitor treatments, and recombinant GFP technology combined with confocal laser scanning microscopy (CLSM) and electron and immune-electron microscopy were routinely employed for investigation of endocytosis in plant cells. However, new approaches such as high-throughput confocal microscopy screens on mutants and proteomic analyses on isolated vesicular compartments and root cells treated with vesicular trafficking inhibitors (both focused on the identification of new endosomal proteins), together with chemical genomics and advanced microscopy approaches such as Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), light sheet-based fluorescence microscopy, and super-resolution microscopy provided a significant amount of new data and these new methods appear as extremely promising tools in this field. The growing interest in the investigation of endocytosis, vesicular transport routes, and corresponding regulatory mechanisms resulted in the exploitation of cell biological, genetic, biochemical, and proteomic approaches. Methods and techniques such as site-directed and T-DNA insertional mutagenesis, RNAi, classical inhibitor treatments, and recombinant GFP technology combined with confocal laser scanning microscopy (CLSM) and electron and immune-electron microscopy were routinely employed for investigation of endocytosis in plant cells. However, new approaches such as high-throughput confocal microscopy screens on mutants and proteomic analyses on isolated vesicular compartments and root cells treated with vesicular trafficking inhibitors (both focused on the identification of new endosomal proteins), together with chemical genomics and advanced microscopy approaches such as Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), light sheet-based fluorescence microscopy, and super-resolution microscopy provided a significant amount of new data and these new methods appear as extremely promising tools in this field. (en)
Title	Update on Methods and Techniques to Study Endocytosis in Plants Update on Methods and Techniques to Study Endocytosis in Plants (en)
skos:prefLabel	Update on Methods and Techniques to Study Endocytosis in Plants Update on Methods and Techniques to Study Endocytosis in Plants (en)
skos:notation	RIV/61989592:15310/12:33140459!RIV13-MSM-15310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED0007/01/01), P(GAP501/11/1764)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Šamaj, Jozef Takáč, Tomáš Šamajová, Olga
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	176095
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/12:33140459
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	proteomics, microscopy, genetics; Plants; Endocytosis; Techniques; Methods (en)
http://linked.open.../riv/klicoveSlovo	Plants Techniques genetics microscopy Methods Endocytosis proteomics
http://linked.open...ontrolniKodProRIV	[A0110575A9E8]
http://linked.open...i/riv/mistoVydani	Heidelberg
http://linked.open...i/riv/nazevZdroje	Endocytosis in Plants
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	336 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Mitogen-activated protein kinase stress signalling in plants: from basic research in Arabidopsis towards biotechnological applications in crops. Centre of the Region Haná for Biotechnological and Agricultural Research
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Takáč, Tomáš Šamaj, Jozef Von Wangenheim, Daniel Stelzer, Ernst Šamajová, Olga
number of pages	37 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-3-642-32463-5_1
http://purl.org/ne...btex#hasPublisher	Springer-Verlag
https://schema.org/isbn	978-3-642-32462-8
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Update on Methods and Techniques to Study Endocytosis in Plants

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