About: On GC/MS in phospholipid research II: Investigation of liposome formation and liposome-water partitioning

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Headspace solid phase microextraction gas chromatography / mass spectrometry was used for determination of critical liposome concentration of soybean phospholipid (asolectin) and liposome water partition coefficient of eugenol. Constant amount of eugenol was incubated with constant volume of asolectin solutions for 12 hours. Peak area corresponding to the eugenol concentration in the headspace and thus to the fraction of free eugenol was recorded in dependence on asolectin concentration. The initial and terminal parts of the experimental dependence were extrapolated with two straight lines whose intersection corresponds to the concentration value (1.097 g/l) remarkably conformable to the value of critical liposome concentration obtained from cyclic voltammetry (1.126 g/l). Liposom water partition coefficient was determined from the measured concentration of free eugenol and known volume of the liposomal phase. Partition coefficient values 300, 297 and 305 were obtained in solutions with asolectin concentration 4, 6 and 8 g/l respectively. Headspace solid phase microextraction gas chromatography / mass spectrometry was used for determination of critical liposome concentration of soybean phospholipid (asolectin) and liposome water partition coefficient of eugenol. Constant amount of eugenol was incubated with constant volume of asolectin solutions for 12 hours. Peak area corresponding to the eugenol concentration in the headspace and thus to the fraction of free eugenol was recorded in dependence on asolectin concentration. The initial and terminal parts of the experimental dependence were extrapolated with two straight lines whose intersection corresponds to the concentration value (1.097 g/l) remarkably conformable to the value of critical liposome concentration obtained from cyclic voltammetry (1.126 g/l). Liposom water partition coefficient was determined from the measured concentration of free eugenol and known volume of the liposomal phase. Partition coefficient values 300, 297 and 305 were obtained in solutions with asolectin concentration 4, 6 and 8 g/l respectively. (en)
Title	On GC/MS in phospholipid research II: Investigation of liposome formation and liposome-water partitioning On GC/MS in phospholipid research II: Investigation of liposome formation and liposome-water partitioning (en)
skos:prefLabel	On GC/MS in phospholipid research II: Investigation of liposome formation and liposome-water partitioning On GC/MS in phospholipid research II: Investigation of liposome formation and liposome-water partitioning (en)
skos:notation	RIV/61989592:15310/11:33118053!RIV12-MSM-15310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ED2.1.00/03.0058), S
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Barták, Petr Skopalová, Jana Součková, Jitka Bednář, Petr Švecová, Hana Čáp, Lubomír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	218029
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/11:33118053
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Asolectin, gas chromatography, mass spectrometry, eugenol, critical liposome concentration, partial coefficient (en)
http://linked.open.../riv/klicoveSlovo	Asolectin critical liposome concentration partial coefficient gas chromatography mass spectrometry eugenol
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[1B29A29445BB]
http://linked.open...i/riv/nazevZdroje	Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Regional Centre of Advanced Technologies and Materials
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	48
http://linked.open...iv/tvurceVysledku	Barták, Petr Bednář, Petr Skopalová, Jana Součková, Jitka Čáp, Lubomír Švecová, Hana
issn	0232-0061
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	On GC/MS in phospholipid research II: Investigation of liposome formation and liposome-water partitioning On GC/MS in phospholipid research II: Investigation of liposome formation and liposome-water partitioning

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