About: Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A fast and precise analysis of the synthetic peptide buserelin in urine using CZE-ESI-MS method has been demonstrated. Formic acid at 50 mmol/L concentration served as backgroud electrolyte in CZE stage and it is compatible with MS detection in positive ionization mode. Two injection modes were tested, i.e. pressure (50 mbar for 5 s) and electrokinetic injection (5 kV for 5 s), of which electrokinetic injection provided better calibration parameters. Buserelin LODs were 0 47 mu g/mL in water and 0 63 mu g/mL in ten times diluted urine samples using pressure injection, while they were 0.32 mu g/mL in water and 0.34 mu g/mL in ten times diluted urine samples using electrokinetic injection Repeatability of buserelin migration times was below 6% (pressure injection mode) and 1% (electrokinetic injection mode). A fast and precise analysis of the synthetic peptide buserelin in urine using CZE-ESI-MS method has been demonstrated. Formic acid at 50 mmol/L concentration served as backgroud electrolyte in CZE stage and it is compatible with MS detection in positive ionization mode. Two injection modes were tested, i.e. pressure (50 mbar for 5 s) and electrokinetic injection (5 kV for 5 s), of which electrokinetic injection provided better calibration parameters. Buserelin LODs were 0 47 mu g/mL in water and 0 63 mu g/mL in ten times diluted urine samples using pressure injection, while they were 0.32 mu g/mL in water and 0.34 mu g/mL in ten times diluted urine samples using electrokinetic injection Repeatability of buserelin migration times was below 6% (pressure injection mode) and 1% (electrokinetic injection mode). (en)
Title	Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry (en)
skos:prefLabel	Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry (en)
skos:notation	RIV/61989592:15310/10:10215687!RIV11-MSM-15310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Maier, Vítězslav Ševčík, Juraj Ranc, Václav Znaleziona, Joanna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	246351
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/10:10215687
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Urine; Mass spectrometry; Electrospray; Capillary electrophoresis; Buserelin (en)
http://linked.open.../riv/klicoveSlovo	Capillary electrophoresis Buserelin Urine Mass spectrometry Electrospray
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[5BECF858B7EE]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Maier, Vítězslav Ranc, Václav Znaleziona, Joanna Ševčík, Juraj Kanianský, Dušan Marák, Jozef Staňová, Andrea
http://linked.open...ain/vavai/riv/wos	000276811000015
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0173-0835
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry

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