About: High-throughput screening technology for monitoring phytohormone production in microalgae

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	New miniaturized techniques for multiplying microalgae and estimating their phytohormone production were developed; in these methods, the strains to be tested are cultivated in microtitre plates, and the phytohormones in suspensions of the cultures are measured by direct ELISAs. Specific and sensitive ELISAs for determining abscisic acid (ABA), indole-3-acetic acid (IAA), cis- and trans-zeatin riboside, isopentenyladenosine (iPR), and other less common cytokinins were developed for this purpose. Polyclonal antibodies used in the ABA and IAA assays were raised against C1- and C1'- conjugates of the compounds with BSA, respectively, and thus were specific for the free acids and their respective C1-derivatives. The use of cytokinin ribosides coupled via their sugar residues to BSA as haptens generally led to antibodies that bound free bases, 9-glycosides and nucleotides, but with high specificity for the corresponding N-6-side chains. Using internal standards, dilution assays, and authentic [H-2] an New miniaturized techniques for multiplying microalgae and estimating their phytohormone production were developed; in these methods, the strains to be tested are cultivated in microtitre plates, and the phytohormones in suspensions of the cultures are measured by direct ELISAs. Specific and sensitive ELISAs for determining abscisic acid (ABA), indole-3-acetic acid (IAA), cis- and trans-zeatin riboside, isopentenyladenosine (iPR), and other less common cytokinins were developed for this purpose. Polyclonal antibodies used in the ABA and IAA assays were raised against C1- and C1'- conjugates of the compounds with BSA, respectively, and thus were specific for the free acids and their respective C1-derivatives. The use of cytokinin ribosides coupled via their sugar residues to BSA as haptens generally led to antibodies that bound free bases, 9-glycosides and nucleotides, but with high specificity for the corresponding N-6-side chains. Using internal standards, dilution assays, and authentic [H-2] an (en)
Title	High-throughput screening technology for monitoring phytohormone production in microalgae High-throughput screening technology for monitoring phytohormone production in microalgae (en)
skos:prefLabel	High-throughput screening technology for monitoring phytohormone production in microalgae High-throughput screening technology for monitoring phytohormone production in microalgae (en)
skos:notation	RIV/61989592:15310/09:00010628!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA206/09/1284), P(GA522/03/0323), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Poulíčková, Aloisie Novák, Ondřej Strnad, Miroslav Turečková, Veronika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	317189
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010628
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	abscisic acid; auxins; cytokinins; enzyme-immunoassays; microalgae; phytohormones (en)
http://linked.open.../riv/klicoveSlovo	abscisic acid cytokinins microalgae phytohormones auxins enzyme-immunoassays
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[F40C29CF65CA]
http://linked.open...i/riv/nazevZdroje	Journal of Phycology
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Investigation of algae and cyanobacteria as producers of biologically active substances Cyanobacterial cell factory: Modeling and experimental validation of models of photosynthetic energy conversion, of growth, and of production.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	45
http://linked.open...iv/tvurceVysledku	Novák, Ondřej Poulíčková, Aloisie Strnad, Miroslav Turečková, Veronika Sedláková, Kamila Jiráskova, Darina
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0022-3646
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	High-throughput screening technology for monitoring phytohormone production in microalgae High-throughput screening technology for monitoring phytohormone production in microalgae High-throughput screening technology for monitoring phytohormone production in microalgae High-throughput screening technology for monitoring phytohormone production in microalgae

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