About: On-line combination of CE and microscopy: An insight into the migration of microorganisms

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	identification of microbial contamination by CE has interested many researchers mainly because of the high speed of CE analysis. However, the CE separation of such big structures brings a lot of questions mainly about the behavior of microorganisms and about the mechanism of separation. In this work, we constructed a simple apparatus where a microscope was used as one detector and a UV detector was used as the second one and we made the comparison of three typical setups for CE of microorganisms. Saccharomyces cerevisiae was chosen as a model microorganism and was analyzed in bare fused-silica capillaries, covalently modified capillaries and dynamically modified capillaries by poly(ethylene oxide) or CTAB. Results showed that the use of CE instrument directly connected with a microscope could be advantageous for the study of separation mechanisms and/or migration behavior. identification of microbial contamination by CE has interested many researchers mainly because of the high speed of CE analysis. However, the CE separation of such big structures brings a lot of questions mainly about the behavior of microorganisms and about the mechanism of separation. In this work, we constructed a simple apparatus where a microscope was used as one detector and a UV detector was used as the second one and we made the comparison of three typical setups for CE of microorganisms. Saccharomyces cerevisiae was chosen as a model microorganism and was analyzed in bare fused-silica capillaries, covalently modified capillaries and dynamically modified capillaries by poly(ethylene oxide) or CTAB. Results showed that the use of CE instrument directly connected with a microscope could be advantageous for the study of separation mechanisms and/or migration behavior. (en)
Title	On-line combination of CE and microscopy: An insight into the migration of microorganisms On-line combination of CE and microscopy: An insight into the migration of microorganisms (en)
skos:prefLabel	On-line combination of CE and microscopy: An insight into the migration of microorganisms On-line combination of CE and microscopy: An insight into the migration of microorganisms (en)
skos:notation	RIV/61989592:15310/09:00010412!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Petr, Jan Ryparová, Olga Maier, Vítězslav Ševčík, Juraj Znaleziona, Joanna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	331664
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010412
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE; Microorganism; Microscope; Saccharomyces cerevisiae (en)
http://linked.open.../riv/klicoveSlovo	Microorganism Microscope Saccharomyces cerevisiae CE
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[87BC713FE7EA]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	30
http://linked.open...iv/tvurceVysledku	Petr, Jan Maier, Vítězslav Znaleziona, Joanna Ševčík, Juraj Ryparová, Olga
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0173-0835
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	On-line combination of CE and microscopy: An insight into the migration of microorganisms On-line combination of CE and microscopy: An insight into the migration of microorganisms On-line combination of CE and microscopy: An insight into the migration of microorganisms On-line combination of CE and microscopy: An insight into the migration of microorganisms

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