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| rdf:type
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| Description
| - Plant hormones cytokinins are degraded by a cytokinin dehydrogenase (CKX, EC 1.5.99.12). There are several isoforms of CKX in each plant encoded by a small highly homologous gene family. These isoforms are differentially targeted in plant cells to achieve specific regulatory functions. Differences in local environments affect CKX activity and dictate differences in modes of biochemical action on cytokinin substrates and electron acceptors. Most of the CKX isoforms are localized in apoplast or vacuole; there is generally only one CKX isoform per plant per species that lacks a translocation signal and presumably functions in cytoplasm. The best characterized CKX from maize is ZmCKX1, known to be secreted into the apoplast, while a gene encoding the non-secreted CKX has not been cloned before. The aims of this work were to clone the gene for non-secreted CKX from maize (nsZmCKX), compare its localization with the apoplastic ZmCKX1 and characterize its biochemical and structural properties. The gene for n
- Plant hormones cytokinins are degraded by a cytokinin dehydrogenase (CKX, EC 1.5.99.12). There are several isoforms of CKX in each plant encoded by a small highly homologous gene family. These isoforms are differentially targeted in plant cells to achieve specific regulatory functions. Differences in local environments affect CKX activity and dictate differences in modes of biochemical action on cytokinin substrates and electron acceptors. Most of the CKX isoforms are localized in apoplast or vacuole; there is generally only one CKX isoform per plant per species that lacks a translocation signal and presumably functions in cytoplasm. The best characterized CKX from maize is ZmCKX1, known to be secreted into the apoplast, while a gene encoding the non-secreted CKX has not been cloned before. The aims of this work were to clone the gene for non-secreted CKX from maize (nsZmCKX), compare its localization with the apoplastic ZmCKX1 and characterize its biochemical and structural properties. The gene for n (en)
- Byl klonován gen pro cytosolickou verzi cytokinin dehydrogenasy, následně byl funkčně popsán. Byla potvrzena jeho lokalizace a srovnána s lokalizaci apoplastického enzymu ZmCKX1 pomocí konfokální mikroskopie. Bylo použito dvou fluorescenčních proteinových značek DsRed a GFP. Pro další biochemickou charakterizaci bude použito rekombinačních proteinů. (cs)
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| Title
| - Apoplastic and non-secreted cytokinin dehydrogenase from maize: homology and diversity
- Apoplastická a nesekretovaná cytokinindehydrogenasa z kukuřice: homologie a diverzita (cs)
- Apoplastic and non-secreted cytokinin dehydrogenase from maize: homology and diversity (en)
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| skos:prefLabel
| - Apoplastic and non-secreted cytokinin dehydrogenase from maize: homology and diversity
- Apoplastická a nesekretovaná cytokinindehydrogenasa z kukuřice: homologie a diverzita (cs)
- Apoplastic and non-secreted cytokinin dehydrogenase from maize: homology and diversity (en)
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| skos:notation
| - RIV/61989592:15310/08:00005616!RIV09-MSM-15310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/08:00005616
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Enzyme localization; Zea mays; Cytokinin oxidase/dehydrogenase; GFP, DsRed; CKX activity assay; Tomato hairy root transformation, Pichia pastoris expression system (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - Studentská vědecká soutěž %22O cenu děkana 2008%22 . Sborník příspěvků.
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
| |
| http://linked.open...iv/tvurceVysledku
| - Frébort, Ivo
- Galuszka, Petr
- Kowalska, Marta
- Šmehilová, Mária
- Bilyeu, Kristin D
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - Univerzita Palackého v Olomouci
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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