About: On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry

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About: On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new method for determination of dissociation constants (K-d) using on-line titration by electrospray ionization mass spectrometry is presented. Unlike in common titration experiments, where a set of discrete solutions with a fixed concentration of host and increasing concentration of guest is measured, here a continuous Gaussian concentration profile of guest, formed by band-broadening dispersion during passage through a long tubing, is utilized. An equation allowing access to dissociation constant values from experimental data fit to a 1:1 binding model was derived and incorporated into an in-house-written computer program for automated data processing. The new method is demonstrated for noncovalent complexes of cinchona alkaloid carbamate chiral selectors with N-dinitrobenzoylleucine enantiomers and a series of cyclodextrins with sulfonated azo dyes. A new method for determination of dissociation constants (K-d) using on-line titration by electrospray ionization mass spectrometry is presented. Unlike in common titration experiments, where a set of discrete solutions with a fixed concentration of host and increasing concentration of guest is measured, here a continuous Gaussian concentration profile of guest, formed by band-broadening dispersion during passage through a long tubing, is utilized. An equation allowing access to dissociation constant values from experimental data fit to a 1:1 binding model was derived and incorporated into an in-house-written computer program for automated data processing. The new method is demonstrated for noncovalent complexes of cinchona alkaloid carbamate chiral selectors with N-dinitrobenzoylleucine enantiomers and a series of cyclodextrins with sulfonated azo dyes. (en)
Title	On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry (en)
skos:prefLabel	On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry (en)
skos:notation	RIV/61989592:15310/07:00004838!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Fryčák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	439515
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/07:00004838
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PROTEIN-LIGAND INTERACTIONS; AMYLOID-BETA-PEPTIDE; MOLECULAR RECOGNITION; BINDING INTERACTIONS; CHIRAL RECOGNITION; ANION-EXCHANGERS; N-METHYLPYRROLE; AMINO-ACIDS; RNA; ENANTIOSELECTIVITY (en)
http://linked.open.../riv/klicoveSlovo	MOLECULAR RECOGNITION AMINO-ACIDS CHIRAL RECOGNITION RNA AMYLOID-BETA-PEPTIDE N-METHYLPYRROLE ENANTIOSELECTIVITY PROTEIN-LIGAND INTERACTIONS ANION-EXCHANGERS BINDING INTERACTIONS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[AAF7A3136358]
http://linked.open...i/riv/nazevZdroje	Analytical Chemistry
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Fryčák, Petr Schug, Kevin A.
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0003-2700
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry

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