About: Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae     Goto   Sponge   NotDistinct   Permalink

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Description
  • Cytokininoxidasa/dehydrogenasa byla produkována kvasinkou Saccharomyces a rekombinantní protein byl purifikován. Byly posány vlastnosti purifikovaného proteinu: pH optimum, substrátová specifita a preference pro různé elektronové akceptory. (cs)
  • Cytokinin dehydrogenase (CKX) is responsible for regulating the endogenous cytokinin content by oxidative removal of the side chain and seven distinct genes, AtCKX1 to AtCKX7, code for the enzyme in Arabidopsis thaliana. The recombinant enzyme AtCKX2 was produced in Saccharomyces cerevisiae after expressing the corresponding gene from a plasmid (pDR197) or following chromosomal integration, under either the constitutive promoter PMA1 or the inducible promoter GAL1. The recombinant protein was purified from yeast culture media using a sequence of chromatographic steps. The purified enzyme had a molecular mass of 61 kDa and a typical flavoprotein spectrum. The specific activity of the enzyme was 87.8 μkat g-1, with isopentenyladenine as a substrate and 2,3-dimethoxy-5-methyl-pbenzoquinone as an electron acceptor. The pH optimum lay between 7.0 and 8.0, depending on the electron akceptor used. AtCKX2 reacts both with isoprenoid and aromatic cytokinins, the activity with isoprenoid cytokinins being t
  • Cytokinin dehydrogenase (CKX) is responsible for regulating the endogenous cytokinin content by oxidative removal of the side chain and seven distinct genes, AtCKX1 to AtCKX7, code for the enzyme in Arabidopsis thaliana. The recombinant enzyme AtCKX2 was produced in Saccharomyces cerevisiae after expressing the corresponding gene from a plasmid (pDR197) or following chromosomal integration, under either the constitutive promoter PMA1 or the inducible promoter GAL1. The recombinant protein was purified from yeast culture media using a sequence of chromatographic steps. The purified enzyme had a molecular mass of 61 kDa and a typical flavoprotein spectrum. The specific activity of the enzyme was 87.8 μkat g-1, with isopentenyladenine as a substrate and 2,3-dimethoxy-5-methyl-pbenzoquinone as an electron acceptor. The pH optimum lay between 7.0 and 8.0, depending on the electron akceptor used. AtCKX2 reacts both with isoprenoid and aromatic cytokinins, the activity with isoprenoid cytokinins being t (en)
Title
  • Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae
  • Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae (en)
  • Funkční exprese a purifikace cytokinin dehydrogenasy z Arabidopsis thaliana (AtCKX2) v Saccharomyces cerevisiae (cs)
skos:prefLabel
  • Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae
  • Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae (en)
  • Funkční exprese a purifikace cytokinin dehydrogenasy z Arabidopsis thaliana (AtCKX2) v Saccharomyces cerevisiae (cs)
skos:notation
  • RIV/61989592:15310/07:00004716!RIV08-MSM-15310___
http://linked.open.../vavai/riv/strany
  • 673-682
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 422726
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/07:00004716
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cytokinin metabolism; cytokinin oxidase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [4E71E2CEF3E9]
http://linked.open...i/riv/nazevZdroje
  • Biologia Plantarum
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 51
http://linked.open...iv/tvurceVysledku
  • Frébort, Ivo
  • Frébortová, Jitka
  • Galuszka, Petr
  • Werner, Tomáš
  • Schmülling, Thomas
http://linked.open...n/vavai/riv/zamer
issn
  • 0006-3134
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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