About: A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of the study was to investigate the, reactive oxygen species (ROS) production in the hypoxanthine-xanthinoxidase (HX-XO), hydrogen peroxide-ferrous sulphate (H2O2-FeSO4) and hydrogen peroxide (H2O2) systems by using various concentrations of ROS scavengers, such as superoxide dismutase (SOD), dimethylthiourea (DMTU) or catalase (CAT). Luminol (0.8 mmol/L) was dissolved in a borate buffer, pH 9.0, and was used as a luminophor in the chemiluminescence (CL) measurements. In the HX-XO system SOD, CAT and DMTU deepened the CL signal, whereas in the H2O2-FeSO4 system, only CAT and DMTU deepened the CL signal, and in the H2O2 system SOD and CAT increased and DMTU deepened the CL signal. Electron spin resonance (ESR) measurements were performed only in the H2O2-FeSO4 system. 5,5-dimethyl-pyrroline-N-oxide (DMPO) was used as a spin trap. According to typical ESR spectra, .OH was produced in this chemical system. The aim of the study was to investigate the, reactive oxygen species (ROS) production in the hypoxanthine-xanthinoxidase (HX-XO), hydrogen peroxide-ferrous sulphate (H2O2-FeSO4) and hydrogen peroxide (H2O2) systems by using various concentrations of ROS scavengers, such as superoxide dismutase (SOD), dimethylthiourea (DMTU) or catalase (CAT). Luminol (0.8 mmol/L) was dissolved in a borate buffer, pH 9.0, and was used as a luminophor in the chemiluminescence (CL) measurements. In the HX-XO system SOD, CAT and DMTU deepened the CL signal, whereas in the H2O2-FeSO4 system, only CAT and DMTU deepened the CL signal, and in the H2O2 system SOD and CAT increased and DMTU deepened the CL signal. Electron spin resonance (ESR) measurements were performed only in the H2O2-FeSO4 system. 5,5-dimethyl-pyrroline-N-oxide (DMPO) was used as a spin trap. According to typical ESR spectra, .OH was produced in this chemical system. (en)
Title	A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species (en)
skos:prefLabel	A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species (en)
skos:notation	RIV/61989592:15310/06:10218731!RIV11-MSM-15310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0512), P(GA524/01/1219), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hrbáč, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	463444
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/06:10218731
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	hydrogen peroxide; hydroxyl radical; superoxide anion radical; chemiluminescence (en)
http://linked.open.../riv/klicoveSlovo	hydroxyl radical chemiluminescence hydrogen peroxide superoxide anion radical
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[82BA08C3F2B8]
http://linked.open...i/riv/nazevZdroje	Luminescence
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Research center of powdered nanomaterials Understanding and modulation of the antioxidative defence mechanisms in oxidative stress
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	21
http://linked.open...iv/tvurceVysledku	Lojek, Antonín Hrbáč, Jan Číž, Milan Komrsková, Daniela
http://linked.open...ain/vavai/riv/wos	000239881900006
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems
issn	1522-7235
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	A comparison of chemical systems for luminometric determination of antioxidant capacity towards individual reactive oxygen species

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