About: Cytokinins and Plant Phenolics: A Cooperative Metabolism

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cytokinin dehydrogenase (CKX) is a key enzyme in regulation of the levels of active cytokinins in plants. We have previously reported that CKX can use products of oxidation of plant phenolics by tyrosinase and peroxidase as acceptors of electrons from oxidized cytokinin and that the phloem sap of maize seedlings contains unknown phenolic precursor of this acceptor. In the present work we investigated a set of substituted phenolic acids for their ability to serve as precursors of electron acceptors in the CKX reaction and analyzed the phloem sap of maize seedlings for the presence of carboxylic phenolic acids using HPLC with a Q-TOF detector. Only p-coumaric, caffeic and chlorogenic acids were converted to effective electron acceptors in the presence of mushroom tyrosinase. Ferulic acid showed weak activity and analogous compounds derived from 4-hydroxybenzoic acid were not active. Compounds effective in in vitro CKX assay were identified in the phloem sap. Cytokinin dehydrogenase (CKX) is a key enzyme in regulation of the levels of active cytokinins in plants. We have previously reported that CKX can use products of oxidation of plant phenolics by tyrosinase and peroxidase as acceptors of electrons from oxidized cytokinin and that the phloem sap of maize seedlings contains unknown phenolic precursor of this acceptor. In the present work we investigated a set of substituted phenolic acids for their ability to serve as precursors of electron acceptors in the CKX reaction and analyzed the phloem sap of maize seedlings for the presence of carboxylic phenolic acids using HPLC with a Q-TOF detector. Only p-coumaric, caffeic and chlorogenic acids were converted to effective electron acceptors in the presence of mushroom tyrosinase. Ferulic acid showed weak activity and analogous compounds derived from 4-hydroxybenzoic acid were not active. Compounds effective in in vitro CKX assay were identified in the phloem sap. (en) Cytokinindehydrogenasa je klíčovým enzymem v regulaci hladin aktivních cytokininů. Fenolické látky oxidované polyfenoloxidasami slouží jako elektronové akceptory tohoto enzymu. Sledovali jsme schopnost aktivovat reakci cytokinindehydrogenasy řadou přirozených fenolických kyselin. (cs)
Title	Cytokinins and Plant Phenolics: A Cooperative Metabolism Cytokinins and Plant Phenolics: A Cooperative Metabolism (en) Cytokininy a fenolové látky v rostlinách: kooperativní metabolismus (cs)
skos:prefLabel	Cytokinins and Plant Phenolics: A Cooperative Metabolism Cytokinins and Plant Phenolics: A Cooperative Metabolism (en) Cytokininy a fenolové látky v rostlinách: kooperativní metabolismus (cs)
skos:notation	RIV/61989592:15310/06:00002621!RIV07-GA0-15310___
http://linked.open.../vavai/riv/strany	379-384
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA522/05/0448)
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Frébort, Ivo Frébortová, Jitka Galuszka, Petr
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	470264
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/06:00002621
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytokinin dehydrogenase; laccase; maize; phenolic acids; phloem (en)
http://linked.open.../riv/klicoveSlovo	phenolic acids laccase cytokinin dehydrogenase maize phloem
http://linked.open...ontrolniKodProRIV	[D7098AE94649]
http://linked.open...i/riv/mistoVydani	Isleworth
http://linked.open...i/riv/nazevZdroje	Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Isolation and identification of physiological electron acceptor of plant cytokinin dehydrogenase and interaction with low molecular mass compounds
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Frébort, Ivo Frébortová, Jitka Galuszka, Petr
number of pages	646 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Global Science Books
https://schema.org/isbn	978-4-903313-00-9
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	Cytokinins and Plant Phenolics: A Cooperative Metabolism Cytokinins and Plant Phenolics: A Cooperative Metabolism

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