About: Batch immunoextraction method for efficient purification of aromatic cytokinins

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A range of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In order to study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complex samples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody had undetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as some synthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificity in enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificity and capacity A range of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In order to study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complex samples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody had undetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as some synthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificity in enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificity and capacity (en)
Title	Batch immunoextraction method for efficient purification of aromatic cytokinins Batch immunoextraction method for efficient purification of aromatic cytokinins (en)
skos:prefLabel	Batch immunoextraction method for efficient purification of aromatic cytokinins Batch immunoextraction method for efficient purification of aromatic cytokinins (en)
skos:notation	RIV/61989592:15310/05:00001951!RIV11-MSM-15310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(IBS4055304), S, Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Doležal, Karel Hényková, Eva Strnad, Miroslav Popa, Igor Lenobel, René Oklešťková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	513576
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/05:00001951
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	batch immunoextraction; 6-benzylaminopurine; antibody (en)
http://linked.open.../riv/klicoveSlovo	6-benzylaminopurine antibody batch immunoextraction
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A61D8E5B5FAC]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A: Symposium Volumes
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	New types of plant growth regulators
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	1100
http://linked.open...iv/tvurceVysledku	Doležal, Karel Hajdúch, Marián Strnad, Miroslav Popa, Igor Lenobel, René Vydra, David Hauserová, Eva Swaczynová, Jana Fuksová, Květoslava
http://linked.open...ain/vavai/riv/wos	000234158600015
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0021-9673
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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