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| rdf:type
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| Description
| - Byla vyvinuta citlivá metoda (HPLC/ESI-MS) pro kvantitativní analýzu přírodních isoprinoidních cytokininů v řasách. (cs)
- The sensitive method for quantitative analysis of natural isoprenoid cytokinins (base, riboside, 9-glucoside, O-glucoside and ribotide) was developed. The method for estimation of 37 naturally occurring cytokinins is based on a baseline chromatographic separation of 20 cytokinins (trans/cis-zeatin, dihydrozeatin, isopentenyladenine, benzyladenine, meta/ortho-topoline groups). Ethanolic extracts containing a mixture of deuterium or 15N labeled standards were purified using a combined DEAE-Sephadex, octadecysilica column and immunoaffinity column based on wide-range specific monoclonal antibodies and analyzed by HPLC linked to a micromass single quadrupole mass spectrometer with an electrospray interface (HPLC/ESI-MS) and a photodiode array detector. All studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M+H]+ with limits of detection between 10 and 50 fmol and with linearity ranges of calibration between 25 fmol and 100 pmol. Analyses of O-glucoside and ribonucl
- The sensitive method for quantitative analysis of natural isoprenoid cytokinins (base, riboside, 9-glucoside, O-glucoside and ribotide) was developed. The method for estimation of 37 naturally occurring cytokinins is based on a baseline chromatographic separation of 20 cytokinins (trans/cis-zeatin, dihydrozeatin, isopentenyladenine, benzyladenine, meta/ortho-topoline groups). Ethanolic extracts containing a mixture of deuterium or 15N labeled standards were purified using a combined DEAE-Sephadex, octadecysilica column and immunoaffinity column based on wide-range specific monoclonal antibodies and analyzed by HPLC linked to a micromass single quadrupole mass spectrometer with an electrospray interface (HPLC/ESI-MS) and a photodiode array detector. All studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M+H]+ with limits of detection between 10 and 50 fmol and with linearity ranges of calibration between 25 fmol and 100 pmol. Analyses of O-glucoside and ribonucl (en)
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| Title
| - Aplication of HPLC/ESI-MS for estimation of cytokinins in algae
- Aplication of HPLC/ESI-MS for estimation of cytokinins in algae (en)
- Využití HPLC/ESI-MS pro stanovení cytokininů v řasách (cs)
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| skos:prefLabel
| - Aplication of HPLC/ESI-MS for estimation of cytokinins in algae
- Aplication of HPLC/ESI-MS for estimation of cytokinins in algae (en)
- Využití HPLC/ESI-MS pro stanovení cytokininů v řasách (cs)
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| skos:notation
| - RIV/61989592:15310/04:00002425!RIV/2005/GA0/153105/N
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/04:00002425
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - aromatic cytokinins;endogenous cytokinins content;isoprenoid cytokinins;microalgae;growth-regulators;identification;algae;cyanobacteria;bioassay;liquid chromatography;mass spectrometry;quantification (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - International Symposium on Microalgae and Seaweed products in Plant/Soil-Systems
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Novák, Ondřej
- Strnad, Miroslav
- Stirk, Wendy A.
- van Staden, Johannes
- Ördög, Vince
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| number of pages
| |
| http://purl.org/ne...btex#hasPublisher
| - European Society of Microalgal Biotechnology
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| http://localhost/t...ganizacniJednotka
| |
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