About: Occurrence of Phytoplasmas in Red and White Currant in the Czech Republic     Goto   Sponge   NotDistinct   Permalink

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Description
  • Pomocí PCR a elektronové mikroskopie byla sledována přítomnost fytoplazem v kultivarech červeného a bílého rybízu, vykazujících plnokvětost. 20 ze 41 testovaných keřů bylo v PCR pozitivních. Detekované fytoplazmy byly na základě RFLP a sekvenování zařazeny do skupin fytoplazmy proliferace jabloní a fytoplazmy žloutenky aster. ESFY byla nejběžnější fytoplasmou identifikovanou ve vzorcích rybízu., nicméně byly detekovány I fytoplazma proliferace jabloní, f. žloutenky aster a f. chřadnutí hrušní. (cs)
  • The presence of phytoplasmas in the shrubs of red ('Vitan', 'Losan', 'Mulka', 'Rubigo', and some of new selections) and white ('Primus') currant exhibiting full blossom symptoms was estimated using nested PCR with primers amplifying phytoplasma 16S rRNA gene and by electron microscopy. In total, 20 of 41 analysed symptomatic bushes tested positive phytoplasma in PCR. Based on the results of RFLP analysis and sequencing the phytoplasma isolates were identified as belonging to the apple proliferation and aster yellows groups. European stone fruit yellows phytoplasma was the most common phytoplasma identified in currants in this study. Furthermore, apple proliferation phytoplasma, aster yellows phytoplasma and pear decline phytoplasma were detected. The presence of phytoplasma bodies in phloem tissue of red currants was confirmed by electron microscopy.
  • The presence of phytoplasmas in the shrubs of red ('Vitan', 'Losan', 'Mulka', 'Rubigo', and some of new selections) and white ('Primus') currant exhibiting full blossom symptoms was estimated using nested PCR with primers amplifying phytoplasma 16S rRNA gene and by electron microscopy. In total, 20 of 41 analysed symptomatic bushes tested positive phytoplasma in PCR. Based on the results of RFLP analysis and sequencing the phytoplasma isolates were identified as belonging to the apple proliferation and aster yellows groups. European stone fruit yellows phytoplasma was the most common phytoplasma identified in currants in this study. Furthermore, apple proliferation phytoplasma, aster yellows phytoplasma and pear decline phytoplasma were detected. The presence of phytoplasma bodies in phloem tissue of red currants was confirmed by electron microscopy. (en)
Title
  • Výskyt fytoplasem z červeného a bílého rybízu v České republice (cs)
  • Occurrence of Phytoplasmas in Red and White Currant in the Czech Republic
  • Occurrence of Phytoplasmas in Red and White Currant in the Czech Republic (en)
skos:prefLabel
  • Výskyt fytoplasem z červeného a bílého rybízu v České republice (cs)
  • Occurrence of Phytoplasmas in Red and White Currant in the Czech Republic
  • Occurrence of Phytoplasmas in Red and White Currant in the Czech Republic (en)
skos:notation
  • RIV/61989592:15310/04:00002424!RIV/2005/GA0/153105/N
http://linked.open.../vavai/riv/strany
  • 119-124
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/02/0040)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 577365
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/04:00002424
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • currant;PCR;16S rRNA;electron microscopy;RFLP;sequencing;ESFY;apple proliferation phytoplasma;aster yellows phytoplasma;pear decline phytoplasma (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [3809870BE43E]
http://linked.open...i/riv/nazevZdroje
  • Acta Horticulturae
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 656
http://linked.open...iv/tvurceVysledku
  • Válová, Pavla
  • Navrátil, P.
  • Fialová, Renata
  • Špak, J.
  • Karešová, R.
  • Přibylová, J.
  • Kubelková, D.
issn
  • 0567-7572
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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