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Description
  • A 10-year-old plant of rhododendron (#Cunningham#s White# PS986) with leaf malformation and variegation was observed in Prague in 1997. This plant was micropropagated, and regenerants with severe, mild and no symptoms were obtained. Phytoplasma infection was detected using nested polymerase chain reaction (PCR) in the original PS986 plant and the symptomatic regenerants but not in nonsymptomatic plants. During 2002, phytoplasmas were also detected in rhododendron hybrids, PS2716 and PS2439, grown in Pruhonice that showed similar symptoms as the plant observed in Prague. In nested PCR, performed as described previously (1), primer pairs R16F1/R16R0 and R16F2/R16R2 were used. The phytoplasmas detected were classified by restriction fragment length polymorphism analysis using R16F2/R16R2 PCR fragments. Following digestion with restriction endonuclease MseI (Promega, Madison, WI), the restriction profiles obtained were identical with the pattern of the stolbur phytoplasma group (16SrXII group) as determin
  • A 10-year-old plant of rhododendron (#Cunningham#s White# PS986) with leaf malformation and variegation was observed in Prague in 1997. This plant was micropropagated, and regenerants with severe, mild and no symptoms were obtained. Phytoplasma infection was detected using nested polymerase chain reaction (PCR) in the original PS986 plant and the symptomatic regenerants but not in nonsymptomatic plants. During 2002, phytoplasmas were also detected in rhododendron hybrids, PS2716 and PS2439, grown in Pruhonice that showed similar symptoms as the plant observed in Prague. In nested PCR, performed as described previously (1), primer pairs R16F1/R16R0 and R16F2/R16R2 were used. The phytoplasmas detected were classified by restriction fragment length polymorphism analysis using R16F2/R16R2 PCR fragments. Following digestion with restriction endonuclease MseI (Promega, Madison, WI), the restriction profiles obtained were identical with the pattern of the stolbur phytoplasma group (16SrXII group) as determin (en)
  • U desetiletých rostlin rhododendronu V Praze byly pozorovány malformace listů. Tyto rostliny byly mikropropagovány a byly získány regeneranti se silnými, slabými anebo žádnými příznaky. Infekcefytoplasmou byla detekována za pomocí nested-PCR v původní rostlině PS986 a v symptomatických regenerantech, nikoli v nesymptomatických rostlinách. V průběhu roku 2002 byla fytoplasma detekována také v symptomatických hybridech pěstovaných v Průhonicích. Detekované fytoplasmy blay klasifikovány pomocí RFLP, restrikční profily odpovídaly fytoplazmě stolburu (16SrXIIskupina). Podle našich informací se jedná o první nález fytoplazmy stolburu u rhodorendronu. (cs)
Title
  • První detekce fytoplazem u rododendronu v České Republice (cs)
  • First Detection of Phytoplasmas in Rhododendron in the Czech Republic
  • First Detection of Phytoplasmas in Rhododendron in the Czech Republic (en)
skos:prefLabel
  • První detekce fytoplazem u rododendronu v České Republice (cs)
  • First Detection of Phytoplasmas in Rhododendron in the Czech Republic
  • First Detection of Phytoplasmas in Rhododendron in the Czech Republic (en)
skos:notation
  • RIV/61989592:15310/04:00002421!RIV/2005/GA0/153105/N
http://linked.open.../vavai/riv/strany
  • 906-906
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/98/0792)
http://linked.open...iv/cisloPeriodika
  • 8
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 564493
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/04:00002421
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • phytoplasma;16S rRNA;PCR;rododendron (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [6AD9BECBA306]
http://linked.open...i/riv/nazevZdroje
  • Plant Disease
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 88
http://linked.open...iv/tvurceVysledku
  • Navrátil, Milan
  • Šedivá, J.
  • Válová, Pavla
  • Kloudová, K.
  • Mertelík, J.
  • Mokrá, V.
  • Vanc, P.
issn
  • 0191-2917
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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