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rdf:type
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Description
| - V článku je kladena otázka, zda se účastní degradace cytokininů v rostlinách fenolové látky? (cs)
- Degradation of cytokinins in plants is controlled by flavoprotein cytokinin dehydrogenase (CKX, EC 1.5.99.12). Cleavage of the cytokinin substrate proceeds via a ternary complex involving covalently bound FAD cofactor and a quinonic electron acceptor 1,2. Maize kernels 3 weeks after pollination and 2-week-old maize seedlings were examined. CKX activity was monitored using a spectrophotometric assay of the product aldehyde with 4-aminophenol3. Histochemical localization of CKX activity was done with PMS/NBT redox-dye system. Activity of laccase was stained with 3,3#-diaminobenzidine. Immunochemical detection of CKX protein was done using specific antibody against the enzyme from maize 4. CKX from maize showed the capability of using oxidation products of guaiacol, catechol, caffeic acid, acetosyringone, some flavonoids and several other compounds as electron acceptors. Further experiments revealed that the electron acceptors may be generated in vivo from plant phenolics by other enzymatic systems, e.g.
- Degradation of cytokinins in plants is controlled by flavoprotein cytokinin dehydrogenase (CKX, EC 1.5.99.12). Cleavage of the cytokinin substrate proceeds via a ternary complex involving covalently bound FAD cofactor and a quinonic electron acceptor 1,2. Maize kernels 3 weeks after pollination and 2-week-old maize seedlings were examined. CKX activity was monitored using a spectrophotometric assay of the product aldehyde with 4-aminophenol3. Histochemical localization of CKX activity was done with PMS/NBT redox-dye system. Activity of laccase was stained with 3,3#-diaminobenzidine. Immunochemical detection of CKX protein was done using specific antibody against the enzyme from maize 4. CKX from maize showed the capability of using oxidation products of guaiacol, catechol, caffeic acid, acetosyringone, some flavonoids and several other compounds as electron acceptors. Further experiments revealed that the electron acceptors may be generated in vivo from plant phenolics by other enzymatic systems, e.g. (en)
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Title
| - Is the degradation of cytokinins in plants assisted by phenolics?
- Is the degradation of cytokinins in plants assisted by phenolics? (en)
- Účastní se degradace cytokininů v rostlinách fenolové látky? (cs)
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skos:prefLabel
| - Is the degradation of cytokinins in plants assisted by phenolics?
- Is the degradation of cytokinins in plants assisted by phenolics? (en)
- Účastní se degradace cytokininů v rostlinách fenolové látky? (cs)
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skos:notation
| - RIV/61989592:15310/04:00002097!RIV/2005/GA0/153105/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA522/03/0979), Z(MSM 153100008)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/04:00002097
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - cytokinin;cytokinin oxidase/dehydrogenase;phenolic compounds (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Drábek, Jiří
- Popelková, Hana
- Frébort, Ivo
- Frébortová, Jitka
- Galuszka, Petr
- Bilyeu, Kristin D.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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