| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Pro stanovení většiny přirozeně se vyskytujících isoprenoidních a aromatických cytokininů v rostlinách jsme vyvinuli novou citlivou metodu. Jejím základem je chromatografická separace 20 rostlinných hormonů - cytokininů (báze, ribosidy a 9-glukosidy) na reverzní fázi ve spojení s hmotnostní detekcí na jednoduchém kvadrupólu (HPLC/MS), za podmínek ionizace elektrosprejem. Pro stanovení O-glukosidů a nukleotidů cytokininů lze tuto metodu rovněž s úspěchem použít po předchozím enzymovém ošetření rostlinných extraktů. Spolehlivost metody byla validována za pomoci směsi deuterovaných cytokininových standardů přidávaných při extrakci rostlinného materiálu. Rostlinný materiál byl po extrakci do Bieleského roztoku přečištěn použitím dvojnásobné ionexové chromatografie (SCX a DEAE Sephadexu) v kombinaci s imunoafinitní chromatografií na gelu s navázanými skupinově selektivními monoklonálními protilátkami proti cytokininům. Přítomnost isoprenoidních a aromatických cytokininů ve studovaných extraktech byla souča (cs)
- A sensitive method for the quantitative analysis of all natural isoprenoid cytokinins in plant material by electrospray single-quadrupole mass spectrometry is presented. A baseline chromatographic separation of 20 non-derivatised naturally occurring cytokinins has been developed. Precise analyses of O-glucoside and ribonucleotide fractions were also performed by the high-performance liquid chromatography-mass spectrometry (HPLC-MS) but run separately from the basic cytokinin metabolites. Using post-column splitting, the flux from narrow-bore (2.1 mm, i.d.) reversed-phase liquid chromatography column was simultaneously introduced into the diode array and mass detector. Optimal conditions, including final flow rate, desolvation temperature, desolvation gas flow, capillary and cone voltage for effective ionisation in the electrospray ion source were found. When low cone voltage (20 V) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M + H](+) wi
- A sensitive method for the quantitative analysis of all natural isoprenoid cytokinins in plant material by electrospray single-quadrupole mass spectrometry is presented. A baseline chromatographic separation of 20 non-derivatised naturally occurring cytokinins has been developed. Precise analyses of O-glucoside and ribonucleotide fractions were also performed by the high-performance liquid chromatography-mass spectrometry (HPLC-MS) but run separately from the basic cytokinin metabolites. Using post-column splitting, the flux from narrow-bore (2.1 mm, i.d.) reversed-phase liquid chromatography column was simultaneously introduced into the diode array and mass detector. Optimal conditions, including final flow rate, desolvation temperature, desolvation gas flow, capillary and cone voltage for effective ionisation in the electrospray ion source were found. When low cone voltage (20 V) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M + H](+) wi (en)
|
| Title
| - Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry
- Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry (en)
- Kvantitavní stanovení cytokininů v rostlinách metodou kapalinové chromatografie-hmotnostní spektrometrie (cs)
|
| skos:prefLabel
| - Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry
- Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry (en)
- Kvantitavní stanovení cytokininů v rostlinách metodou kapalinové chromatografie-hmotnostní spektrometrie (cs)
|
| skos:notation
| - RIV/61989592:15310/03:00002990!RIV07-GA0-15310___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA522/01/0275), P(GA522/03/0323), Z(AV0Z5038910), Z(MSM 153100008)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/03:00002990
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - liquid chromatography; mass spectrometry; cytokinins; ELISA; tobacco; poplar; tissue; 6-benzylaminopurine; quantification; derivatives; metabolism (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Doležal, Karel
- Novák, Ondřej
- Strnad, Miroslav
- Lenobel, René
- Tarkowski, Petr
- Pěchová, Danuše
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)