About: Xanthine dehydrogenase from pea seedlings: a member of plant molybdenum oxidoreductase family

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Xanthine dehydrogenase (XDH, EC 1.1.1.204) was purified to homogeneity from etiolated pea (Pisum sativum conv. speciosum) seedlings. The procedure involved initial purification with precipitants followed by two low pressure chromatographic steps. The partially purified enzyme was further subjected to FPLC on Superdex and Uno Q columns and to affinity-interaction chromatography on Affi-Gel Blue. Purity of the final enzyme preparation was checked by SDS-PAGE. Pea XDH forms a dimer of 2 × 150 kDa in the native state and is an acidic protein with pI 5.3. The enzyme shows quite stringent substrate specificity; only xanthine and hypoxantine are oxidized at a high reaction rate, some aldehydes such as indole-3-acetaldehyde are converted as well, but at rates lower than 3%. The enzyme was strongly inhibited by allopurinol, a typical inhibitor of molybdenum cofactor-containing enzymes, and less strongly by adenine and some cytokinins with aromatic side chain. N-terminal amino acid sequence of the pea XDH shows Xanthine dehydrogenase (XDH, EC 1.1.1.204) was purified to homogeneity from etiolated pea (Pisum sativum conv. speciosum) seedlings. The procedure involved initial purification with precipitants followed by two low pressure chromatographic steps. The partially purified enzyme was further subjected to FPLC on Superdex and Uno Q columns and to affinity-interaction chromatography on Affi-Gel Blue. Purity of the final enzyme preparation was checked by SDS-PAGE. Pea XDH forms a dimer of 2 × 150 kDa in the native state and is an acidic protein with pI 5.3. The enzyme shows quite stringent substrate specificity; only xanthine and hypoxantine are oxidized at a high reaction rate, some aldehydes such as indole-3-acetaldehyde are converted as well, but at rates lower than 3%. The enzyme was strongly inhibited by allopurinol, a typical inhibitor of molybdenum cofactor-containing enzymes, and less strongly by adenine and some cytokinins with aromatic side chain. N-terminal amino acid sequence of the pea XDH shows (en)
Title	Xanthine dehydrogenase from pea seedlings: a member of plant molybdenum oxidoreductase family Xanthine dehydrogenase from pea seedlings: a member of plant molybdenum oxidoreductase family (en)
skos:prefLabel	Xanthine dehydrogenase from pea seedlings: a member of plant molybdenum oxidoreductase family Xanthine dehydrogenase from pea seedlings: a member of plant molybdenum oxidoreductase family (en)
skos:notation	RIV/61989592:15310/02:00001638!RIV/2003/MSM/153103/N
http://linked.open.../vavai/riv/strany	393-400
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 153100008), Z(MSM 153100010)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Peč, Pavel Šebela, Marek Frébort, Ivo Frébortová, Jitka Galuszka, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	670853
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/02:00001638
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Aldehyde oxidase; Molybdenum cofactor; Sequence; Pea; Purification; Xanthine dehydrogenase; Xanthine oxidase (en)
http://linked.open.../riv/klicoveSlovo	Purification Pea Xanthine dehydrogenase Sequence Aldehyde oxidase Molybdenum cofactor Xanthine oxidase
http://linked.open...odStatuVydavatele	FR - Francouzská republika
http://linked.open...ontrolniKodProRIV	[E219ECAFD059]
http://linked.open...i/riv/nazevZdroje	Plant Physiology and Biochemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	40
http://linked.open...iv/tvurceVysledku	Šebela, Marek Frébort, Ivo Frébortová, Jitka Galuszka, Petr Sauer, Pavel Peč, Pavel Jacobsen, Susanne
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100008 http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100010
issn	0981-9428
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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