About: Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igkappa in insect cells

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About: Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igkappa in insect cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The human UDP-N-acetyl-alfa-d-galactosamine:polypeptide N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) is one of the key enzymes that initiate synthesis of hinge-region O-linked glycans of human immunoglobulin A1 (IgA1). We designed secreted soluble form of human GalNAc-T2 as a fusion protein containing mouse immunoglobulin light chain kappa secretory signal and expressed it using baculovirus and mammalian expression vectors. The recombinant protein was secreted by insect cells Sf9 and human HEK 293T cells in the culture medium. The protein was purified from the media using affinity Ni-NTA chromatography followed by stabilization of purified protein in 50. mM Tris-HCl buffer at pH 7.4. The human UDP-N-acetyl-alfa-d-galactosamine:polypeptide N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) is one of the key enzymes that initiate synthesis of hinge-region O-linked glycans of human immunoglobulin A1 (IgA1). We designed secreted soluble form of human GalNAc-T2 as a fusion protein containing mouse immunoglobulin light chain kappa secretory signal and expressed it using baculovirus and mammalian expression vectors. The recombinant protein was secreted by insect cells Sf9 and human HEK 293T cells in the culture medium. The protein was purified from the media using affinity Ni-NTA chromatography followed by stabilization of purified protein in 50. mM Tris-HCl buffer at pH 7.4. (en)
Title	Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igkappa in insect cells Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igkappa in insect cells (en)
skos:prefLabel	Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igkappa in insect cells Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igkappa in insect cells (en)
skos:notation	RIV/61989592:15110/12:10224923!RIV13-MZ0-15110___
http://linked.open...avai/predkladatel	Lékařská fakulta
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP302/10/1055), P(LH11046), P(NT11081), Z(MSM6198959223)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	RAŠKA, Milan Horynová, Milada
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	162477
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/12:10224923
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GalNAc-T2 (en)
http://linked.open.../riv/klicoveSlovo	GalNAc-T2
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[54D2279D07B0]
http://linked.open...i/riv/nazevZdroje	Protein Expression and Purification
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of IgA1 glycosylation in IgA nephropathy The study of interactions among IgA1-glycosylating enzymes in IgA nephropathy Molecular approaches for elimination or inactivation of pathogenic circulating immune complexes in IgA nephropathy and Henoch-Schoenlein purpura.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	81
http://linked.open...iv/tvurceVysledku	Novák, Jan Raška, Milan Stuchlová Horynová, Milada Hall, Stacy Renfrow, Matthew Takahashi, Kazuo
http://linked.open...ain/vavai/riv/wos	000298073600005
http://linked.open...n/vavai/riv/zamer	Nové možnosti diagnostiky a imunomodulace u infekčních onemocnění a imunopatologických stavů
issn	1046-5928
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.pep.2011.10.006
http://localhost/t...ganizacniJednotka	15110
is http://linked.open...avai/riv/vysledek of	Production of N-acetylgalactosaminyl-transferase 2 (GalNAc-T2) fused with secretory signal Igkappa in insect cells

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