About: Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma 1 and -gamma 2 mRNAs     Goto   Sponge   NotDistinct   Permalink

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  • RT-PCR study was conducted to reveal the effects of normal and high glucose concentration without or with oleate (0.3 mmol/l) on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-)alfa, -gama1, -gama2, and peroxisome proliferator activated receptor-gama coactivator- (PGC-)1alfa and -1beta in HepG2 cells under low-serum condition. Significant decrease in PPAR-gama1 and PGC-1alfa mRNA levels to about 50 % was observed during the first 4 h incubation period. During the next 4 h period, both PPAR-gama1 and PGC-1alfa mRNAs were partly but significantly restored in high glucose batches. The presence of the transcriptional inhibitor actinomycin D revealed a signifiant protective effect of excess glucose on mature PPAR-gama1 and PGC-1alfa mRNAs. Furthermore, PPAR-gama1 and -gama2 mRNAs were differentially superinduced 1.2-2.5 fold in cells upon the administration of the translational inhibitor cycloheximide.
  • RT-PCR study was conducted to reveal the effects of normal and high glucose concentration without or with oleate (0.3 mmol/l) on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-)alfa, -gama1, -gama2, and peroxisome proliferator activated receptor-gama coactivator- (PGC-)1alfa and -1beta in HepG2 cells under low-serum condition. Significant decrease in PPAR-gama1 and PGC-1alfa mRNA levels to about 50 % was observed during the first 4 h incubation period. During the next 4 h period, both PPAR-gama1 and PGC-1alfa mRNAs were partly but significantly restored in high glucose batches. The presence of the transcriptional inhibitor actinomycin D revealed a signifiant protective effect of excess glucose on mature PPAR-gama1 and PGC-1alfa mRNAs. Furthermore, PPAR-gama1 and -gama2 mRNAs were differentially superinduced 1.2-2.5 fold in cells upon the administration of the translational inhibitor cycloheximide. (en)
Title
  • Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma 1 and -gamma 2 mRNAs
  • Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma 1 and -gamma 2 mRNAs (en)
skos:prefLabel
  • Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma 1 and -gamma 2 mRNAs
  • Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma 1 and -gamma 2 mRNAs (en)
skos:notation
  • RIV/61989592:15110/10:10215624!RIV11-MSM-15110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(OC 153), Z(MSM6198959205)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 257973
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15110/10:10215624
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • HepG2 cells; mRNA stability; superinduction; PPAR-gama coactivator; peroxisome proliferator-activated receptor (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • PL - Polská republika
http://linked.open...ontrolniKodProRIV
  • [DD24E03C43E4]
http://linked.open...i/riv/nazevZdroje
  • Acta Biochimica Polonica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 57
http://linked.open...iv/tvurceVysledku
  • Veselý, Jaroslav
  • Rypka, Miroslav
http://linked.open...n/vavai/riv/zamer
issn
  • 0001-527X
number of pages
http://localhost/t...ganizacniJednotka
  • 15110
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