About: Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism.

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About: Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The sensitivity of cancer cells which exhibit multidrug resistance phenotype to A3 adenosine receptor (A3AR) agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide (IB-MECA) was studied. Methods: To establish direct relationship between P-glycoprotein (P-gp, ABCB1, MDR1) and IB-MECA, a straightforward method for precise estimation of intracellular level of this A3AR agonist was developed. Results: We subjected three human leukemia cell lines HL-60, K562, and K562/HHT to the treatment with micromolar concentration of IB-MECA. Despite the fact that all cell lines used expressed A3AR, there was a large difference in sensitivity to IB-MECA. While HL-60 and K562 cells were almost equally sensitive, the K562/HHT cells, which exhibit a multidrug resistance phenotype due to overexpression of P-gp, were significantly more resistant. We found that the intracellular level of IB-MECA in K562/HHT cells was approximately ten times lower than those in HL-60 or K562 cells. The sensitivity of cancer cells which exhibit multidrug resistance phenotype to A3 adenosine receptor (A3AR) agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide (IB-MECA) was studied. Methods: To establish direct relationship between P-glycoprotein (P-gp, ABCB1, MDR1) and IB-MECA, a straightforward method for precise estimation of intracellular level of this A3AR agonist was developed. Results: We subjected three human leukemia cell lines HL-60, K562, and K562/HHT to the treatment with micromolar concentration of IB-MECA. Despite the fact that all cell lines used expressed A3AR, there was a large difference in sensitivity to IB-MECA. While HL-60 and K562 cells were almost equally sensitive, the K562/HHT cells, which exhibit a multidrug resistance phenotype due to overexpression of P-gp, were significantly more resistant. We found that the intracellular level of IB-MECA in K562/HHT cells was approximately ten times lower than those in HL-60 or K562 cells. (en)
Title	Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism. Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism. (en)
skos:prefLabel	Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism. Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism. (en)
skos:notation	RIV/61989592:15110/10:10213496!RIV11-MZ0-15110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(NR9482), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	MLEJNEK, Petr DOLEŽEL, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	263471
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/10:10213496
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	mechanism; intracellular; involvement; possible; cells:; leukemia; human; N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide; agonist; receptor; adenosine; apoptosis; Induction (en)
http://linked.open.../riv/klicoveSlovo	Induction human leukemia agonist apoptosis possible receptor N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide cells: intracellular mechanism involvement adenosine
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[E3F8494C2B3E]
http://linked.open...i/riv/nazevZdroje	Acta Physiologica
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	P-glycoprotein and its possible contribution to development of resistance to Imatinib in Bcr-Abl expressing cells
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	199
http://linked.open...iv/tvurceVysledku	DOLEŽEL, Petr MLEJNEK, Petr
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1748-1708
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	15110
is http://linked.open...avai/riv/vysledek of	Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism. Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism. Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism.

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