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| Description
| - V této práci jsme stanovili účinky dehydroepiandrosteronu (DHEA) na intracelulární hladiny mRNA receptorů aktivovanými peroxisomálními proliferátory (PPAR) a PPAR-γ koaktivátorů v buněčné linii HepG2 za pomoci metody kvantitativní real-time RT-PCR. Ovlivnění 100 μmol/l DHEA po dobu 2-20 hodin vedlo ke zvýšení hladin sledovaných mRNA s časem. Po 20 hodinách jsme pozorovali zvýšení mRNA PPAR-α, -γ1 a ?γ2 i koaktivátorů PGC-1α a -1β na 157, 161, 155, 656 a 475 % (p < 0,05) oproti kontrole. Ovlivnění aktinomycinem D po dobu 2,5 ? 8 hodin odhalilo signifikantní stabilizační účinek DHEA na mRNA PPAR-γ1 a PGC-1α v obou časových intervalech. 8-hodinová inkubace s aktinomycinem D také vyvolala mírný, ale signifikantní stabilizační účinek na PGC-1β. Z výsledků vyplývá, že DHEA může řídit přeměnu těchto transkriptů. Po 20-hodinovém působeni cykloheximidu došlo k signifikantnímu poklesu bazálních hladin mRNA PPAR-α a PGC-1α, zatímco hladiny mRNA PPAR (cs)
- To investigate the effect of dehydroepiandrosterone (DHEA) on intracellular mRNA levels of peroxisome proliferator-activated receptors (PPARs) and PPAR-gamma coactivators (PGCs), we conducted a quantitative real-time RT-PCR study using HepG2 cells. Treatment with 100 micromol/l DHEA for 2-20 h caused a time-dependent elevation of mRNA levels in the cells. Upon 20 h, PPAR-alpha, -gamma1, and -gamma2 mRNAs and PGC-1alpha and -1beta mRNAs increased to 157, 161, 155, 656, and 475% of control levels, respectively (p < 0.05 each). Treatment with actinomycin D for 2.5-8 h revealed a significant stabilization effect of DHEA on PPAR-gamma1 and PGC-1alpha mRNAs at both 2.5 and 8 h incubation periods and a mild but significant stabilization effect on PGC-1beta mRNA at the 8 h incubation period suggesting that DHEA can modulate turnover of these mRNA transcripts. Basal mRNA levels of PPAR-alpha and PGC-1alpha were significantly suppressed upon 20 h treatment with cycloheximide, while those of PPAR-gamma1, -gam
- To investigate the effect of dehydroepiandrosterone (DHEA) on intracellular mRNA levels of peroxisome proliferator-activated receptors (PPARs) and PPAR-gamma coactivators (PGCs), we conducted a quantitative real-time RT-PCR study using HepG2 cells. Treatment with 100 micromol/l DHEA for 2-20 h caused a time-dependent elevation of mRNA levels in the cells. Upon 20 h, PPAR-alpha, -gamma1, and -gamma2 mRNAs and PGC-1alpha and -1beta mRNAs increased to 157, 161, 155, 656, and 475% of control levels, respectively (p < 0.05 each). Treatment with actinomycin D for 2.5-8 h revealed a significant stabilization effect of DHEA on PPAR-gamma1 and PGC-1alpha mRNAs at both 2.5 and 8 h incubation periods and a mild but significant stabilization effect on PGC-1beta mRNA at the 8 h incubation period suggesting that DHEA can modulate turnover of these mRNA transcripts. Basal mRNA levels of PPAR-alpha and PGC-1alpha were significantly suppressed upon 20 h treatment with cycloheximide, while those of PPAR-gamma1, -gam (en)
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| Title
| - Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells
- Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells (en)
- Účinky dehydroepiandrosteronu na úroveň mRNA PPAR a jejich kokoaktivátorů v lidských hepatomových buňkách HepG2 (cs)
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| skos:prefLabel
| - Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells
- Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells (en)
- Účinky dehydroepiandrosteronu na úroveň mRNA PPAR a jejich kokoaktivátorů v lidských hepatomových buňkách HepG2 (cs)
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| skos:notation
| - RIV/61989592:15110/07:00004435!RIV08-MSM-15110___
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(OC 153), S, Z(MSM6198959205)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/07:00004435
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Dehydroepiandrosterone; Peroxisome proliferator-activated receptor; PPAR-gamma coactivator; mRNA stability; HepG2 cells (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - General Physiology and Biophysics
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Veselý, Jaroslav
- Uherková, Lenka
- Rypka, Miroslav
- Bogdanová, Kateřina
- Poczatková, Hana
- Riegrová, Dagmar
- Červenková, Kateřina
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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