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| Description
| - Buněčná signalizace glukokortikoidním receptorem a aryl uhlovodíkovým receptorem je narušena látkami interferujícími s mikrotubuly (MIA). Následkem je down-regulace enzymů metabolizujících léčiva a lékové interakce. Zde jsme zkoumali účinky kyseliny all-trans retinové (ATRA) a MIA (tj. kolchicinu, nokodazolu, taxolu) na regulaci genů receptorů pro kyselinu retinovou (RAR) v primárních kulturách potkaních hepatocytů. ATRA způsobila pokles RARa a RARg mRNA (pokles 23% a 41%), zatímco obsah RARb mRNA byl zvýšen (4,3 krát). Všechny MIA způsobily koncentračně závislý pokles exprese RAR receptorů; účinnost MIA rostla v pořadí NOC<COL<TAX a míra inhibice rostla v pořadí RARa<RARg<RARb. Obsah RARa proteinu byl snížen působením MIA a ATRA. Účinky ATRA byly zvráceny inhibitorem proteasomu MG132, což ukazuje ligand-dependentní degradaci RARa receptoru. Na druhou stranu, účinky MIA byly nezávislé na proteasomu a pokles RARa byl způsoben spíše snížení genové exprese. Sledovali jsme transkripční aktivit (cs)
- Cellular signaling by glucocorticoid receptor and aryl hydrocarbon receptor is restricted by microtubules interfering agents (MIAs). This leads to down-regulation of drug metabolizing enzymes and drug interactions. Here we investigated the effects of all-trans-retinoic acid (ATRA) and MIAs, i.e. colchicine, nocodazole and taxol on the regulation of retinoic acid receptor (RAR) genes in primary cultures of rat hepatocytes. ATRA (1 μM) down-regulated RARα and RARγ mRNAs (decrease 23% and 41%, respectively) whereas it up-regulated RARβ mRNA (4.3-fold induction). All MIAs diminished the expression of RARs in dose-dependent manner; the potency of MIAs increased in order NOC < COL < TAX and the extent of inhibition increased in order RARα < RARγ < RARβ. The levels of RARα protein were decreased by both MIAs and ATRA. The effects of ATRA were reversed by proteasome inhibitor MG-132, implying ligand-dependent RARα degradation. In contrast, the effects o
- Cellular signaling by glucocorticoid receptor and aryl hydrocarbon receptor is restricted by microtubules interfering agents (MIAs). This leads to down-regulation of drug metabolizing enzymes and drug interactions. Here we investigated the effects of all-trans-retinoic acid (ATRA) and MIAs, i.e. colchicine, nocodazole and taxol on the regulation of retinoic acid receptor (RAR) genes in primary cultures of rat hepatocytes. ATRA (1 μM) down-regulated RARα and RARγ mRNAs (decrease 23% and 41%, respectively) whereas it up-regulated RARβ mRNA (4.3-fold induction). All MIAs diminished the expression of RARs in dose-dependent manner; the potency of MIAs increased in order NOC < COL < TAX and the extent of inhibition increased in order RARα < RARγ < RARβ. The levels of RARα protein were decreased by both MIAs and ATRA. The effects of ATRA were reversed by proteasome inhibitor MG-132, implying ligand-dependent RARα degradation. In contrast, the effects o (en)
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| Title
| - Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes
- Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes (en)
- Exprese, stabilita proteinu a transkripční aktivity receptorů pro kyselinu retinovou v primárních potkaních hepatocytech jsou ovlivněny látkami narušujícími mikrotubuly a all-trans retinovou kyselinou (cs)
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| skos:prefLabel
| - Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes
- Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes (en)
- Exprese, stabilita proteinu a transkripční aktivity receptorů pro kyselinu retinovou v primárních potkaních hepatocytech jsou ovlivněny látkami narušujícími mikrotubuly a all-trans retinovou kyselinou (cs)
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| skos:notation
| - RIV/61989592:15110/07:00003561!RIV08-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GP303/04/P074), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/07:00003561
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Retinoic acid receptors; Cellular signaling; Microtubules; Rat hepatocytes (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Molecular and Cellular Endocrinology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dvořák, Zdeněk
- Ulrichová, Jitka
- Vrzal, Radim
- Macejová, Dana
- Brtko, Július
- Ondková, Slavomíra
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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