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| Description
| - Možné interakce mezi kvartérním bezofenanthridinovým alkaloidem sanguinarinem (SA) a regulační drahou aryluhlovodíkový receptor/cytochrom P450 CYP1A (AhR/CYP1A) jsou předmětem neutuchajících diskuzí. Předpokládá se úloha CYP1A enzymů a AhR receptoru v toxicitě SA. V této práci jsme testovali, zda vybrané induktory CYP enzymů modulují cytotoxicitu SA v primárních kulturách potkaních hepatocytů. Buňky byly pre-inkubovány 48 hod s dioxinem (TCDD; 5 nM), fenobarbitalem (PB; 500 mM) nebo DMSO před vlastní inkubací s SA. Samotný SA vykazoval časově a koncentračně závislou cytotoxicitu, monitorováno jako aktivita laktát dehydrogenasy v médiu a MTT test. Preinkubace hepatocytů s TCDD nebo PB signifikantně snížila cytotoxicitu SA; účinky byly výraznější v nižších koncentracích SA a v kratších periodách inkubace. Předpokládali jsme, že zymy CYP1A jsou zapojeny do vymizení cytotoxicity SA. Bylo překvapivé, že inkubace buněk se SA společně s furafylinem, inhibitorem CYP1A enzymů, ještě více snížila cytotoxicitu S (cs)
- Putative interactions between quaternary benzo[c]phenanthridine alkaloid sanguinarine (SA) and aryl hydrocarbon receptor/cytochrome P450 CYP1A (AhR/CYP1A) regulatory pathway are the subject of perpetual disputations. The role of CYP1A enzymes and AhR receptor in SA cytotoxicity was anticipated. In this paper, we tested, whether selected inducers of CYP enzymes modulate cytotoxicity of SA in primary cultures of rat hepatocytes. Cells were challenged 48h with dioxin (TCDD; 5nM), phenobarbital (PB; 500microM) or DMSO prior to the treatment with SA. SA itself displayed time- and dose-dependent cytotoxicity as revealed by lactate dehydrogenase leakage into the medium and MTT test. Pre-treatment of hepatocytes with TCDD and/or PB significantly attenuated SA cytotoxicity, the effects being more pronounced at lower concentrations of SA and shorter periods of incubation. We assumed involvement of CYP1A enzymes in diminution of SA cytotoxicity. Surprisingly, co-treatment with SA and furafylline, an inhibitor of
- Putative interactions between quaternary benzo[c]phenanthridine alkaloid sanguinarine (SA) and aryl hydrocarbon receptor/cytochrome P450 CYP1A (AhR/CYP1A) regulatory pathway are the subject of perpetual disputations. The role of CYP1A enzymes and AhR receptor in SA cytotoxicity was anticipated. In this paper, we tested, whether selected inducers of CYP enzymes modulate cytotoxicity of SA in primary cultures of rat hepatocytes. Cells were challenged 48h with dioxin (TCDD; 5nM), phenobarbital (PB; 500microM) or DMSO prior to the treatment with SA. SA itself displayed time- and dose-dependent cytotoxicity as revealed by lactate dehydrogenase leakage into the medium and MTT test. Pre-treatment of hepatocytes with TCDD and/or PB significantly attenuated SA cytotoxicity, the effects being more pronounced at lower concentrations of SA and shorter periods of incubation. We assumed involvement of CYP1A enzymes in diminution of SA cytotoxicity. Surprisingly, co-treatment with SA and furafylline, an inhibitor of (en)
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| Title
| - Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital
- Cytotoxicita sanguinarinu v primárních potkaních hepatocytech je snížena dioxinem a fenobarbitalem (cs)
- Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital (en)
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| skos:prefLabel
| - Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital
- Cytotoxicita sanguinarinu v primárních potkaních hepatocytech je snížena dioxinem a fenobarbitalem (cs)
- Cytotoxicity of sanguinarine in primary rat hepatocytes is attenuated by dioxin and phenobarbital (en)
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| skos:notation
| - RIV/61989592:15110/06:00003478!RIV07-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/06:00003478
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Sanguinarine; Cytochrome P450; Induction; Cytotoxicity (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dvořák, Zdeněk
- Ulrichová, Jitka
- Anzenbacherová, Eva
- Šimánek, Vilím
- Zdařilová, Adéla
- Šperlíková, Lucie
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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