About: Batch immunoextraction method for an effective purification of aromatic cytokinins.

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About: Batch immunoextraction method for an effective purification of aromatic cytokinins. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Přirozené benzylaminopuriny přitomné v rostlinách mají vysokou bilogickou aktivitu. Další byly syntetizované jak npř. 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), které vykazují vysokou protinádorovou aktivitu v in vitro podmínkách. (cs) Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand capacity for aro Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand capacity for aro (en)
Title	Batch immunoextraction method for an effective purification of aromatic cytokinins. Batch immunoextraction method for an effective purification of aromatic cytokinins. (en) Série imunoextrakčních metod na efektivní purifikaci aromatických cytokininů. (cs)
skos:prefLabel	Batch immunoextraction method for an effective purification of aromatic cytokinins. Batch immunoextraction method for an effective purification of aromatic cytokinins. (en) Série imunoextrakčních metod na efektivní purifikaci aromatických cytokininů. (cs)
skos:notation	RIV/61989592:15110/05:00001951!RIV06-MSM-15110___
http://linked.open.../vavai/riv/strany	116-125
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Vydra, David Doležal, Karel Hényková, Eva Strnad, Miroslav Popa, Igor Hajdúch, Marián Lenobel, René Oklešťková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	513575
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/05:00001951
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	antibody; batch immunoextraction; 6-benzylaminopurine (en)
http://linked.open.../riv/klicoveSlovo	6-benzylaminopurine antibody batch immunoextraction
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C31E3776BAE1]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A: Symposium Volumes
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	1100
http://linked.open...iv/tvurceVysledku	Doležal, Karel Hajdúch, Marián Strnad, Miroslav Popa, Igor Lenobel, René Vydra, David Hauserová, Eva Swaczynová, Jana Fuksová, Květoslava
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0021-9673
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	15110
is http://linked.open...avai/riv/vysledek of	Batch immunoextraction method for an effective purification of aromatic cytokinins.

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