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Description
| - V této práci jsme studovali, zda jsou mikrotubuly a %22heat shock%22 protein 90 (Hsp90) v buňkách HL-60 diferencovaných dimethylsulfoxidem zapojeny do oxidativního vzplanutí aktivovaného 12-O-myristoylforbol-13-acetátem (PMA) a peptidem N-formyl-Met-Leu-Phe (fMLP). Výsledky ukázaly, že látky interagující s mikrotubuly, paklitaxel (1-5 #M), kolchicin (1-100 #M), nokodazol (1-20 #M) a vinkristin (1-50 #M), neovlivňují oxidativní vzplanutí aktivované PMA ani fMLP. Naproti tomu radicikol, inhibitor proteinu Hsp90, inhiboval časově a koncentračně závislým způsobem oxidativní vzplanutí aktivované fMLP, přičemž hodnota IC50 měla při preinkubaci 30 min hodnotu 16,5 # 3,5 #M. Z výsledků usuzujeme, že oxidativní vzplanutí v diferencovaných buňkách HL-60 aktivované PMA i fMLP je nezávislé na mikrotubulech a že vzplanutí vyvolané fMLP vyžaduje aktivitu Hsp90. (cs)
- In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 microM), colchicine (1-100 microM), nocodazole (1-20 microM), and vincristine (1-50 microM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 +/- 3.5 microM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity.
- In this study we examined whether microtubules and heat shock protein 90 (Hsp90) are involved in phorbol myristate acetate (PMA) and N-formyl-Met-Leu-Phe (fMLP)-induced oxidative burst in DMSO-differentiated HL-60 cells. Our results showed that microtubule interfering agents, paclitaxel (1-5 microM), colchicine (1-100 microM), nocodazole (1-20 microM), and vincristine (1-50 microM), did not affect either PMA or fMLP-induced oxidative burst. In contrast, radicicol, an inhibitor of Hsp90, inhibited fMLP-induced oxidative burst in time and concentration-dependent manner where IC50 value for 30 min pre-incubation was 16.5 +/- 3.5 microM radicicol. We conclude that both PMA and fMLP-induced oxidative burst in DMSO-differentiated HL-60 cells is microtubule-independent while the latter requires Hsp90 activity. (en)
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Title
| - N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules.
- N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules. (en)
- Oxidativní vzplanutí aktivované peptidem N-formyl-Met-Leu-Phe vyžaduje v buňkách HL-60 diferencovaných dimethylsulfoxidem aktivní Hsp90, ale nevyžaduje neporušené mikrotubuly (cs)
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skos:prefLabel
| - N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules.
- N-formyl-Met-Leu-Phe-induced oxidative burst in DMSO-differentiated HL-60 cells requires active Hsp90, but not intact microtubules. (en)
- Oxidativní vzplanutí aktivované peptidem N-formyl-Met-Leu-Phe vyžaduje v buňkách HL-60 diferencovaných dimethylsulfoxidem aktivní Hsp90, ale nevyžaduje neporušené mikrotubuly (cs)
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skos:notation
| - RIV/61989592:15110/04:00002001!RIV06-MSM-15110___
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/04:00002001
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Radicicol; Hsp90; Microtubules; Oxidative burst; HL-60 (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Vrba, Jiří
- Modrianský, Martin
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http://linked.open...n/vavai/riv/zamer
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issn
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http://localhost/t...ganizacniJednotka
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