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| Description
| - Nanomaterials (NMs) have become crucial for many applications promising significant improvement in various aspects of quality of life. Indisputable benefits of NMs, however, might be discredited by concerns about potential adverse health effects arising from unique properties related to the nanosize. Safety assessment of NMs is complicated by the fact that conventional methods are insufficient for evaluating their toxicity. Although new methods adapted for NMs are being extensively introduced by toxicologists, the lack of standardization causes inconsistency in existing toxicity data making it impossible to draw general conclusions about NM toxicity. QualityNano is a current European FP7 project aimed at developing and implementing best practice and quality in nanosafety assessment. As one of the participating laboratories, we evaluated cytotoxic potential of two reference NMs (non-cytotoxic 40nm carboxy-functionalized and cytotoxic 50nm amino-functionalized polystyrene nanoparticles) in lung adenocarcinoma A549 cell line using QualityNano Standard Operation Procedure for MTS Cell Viability Assay. Results of two performers showed good intra-laboratory concordance and obtained data were provided for inter-laboratory variation assessment which is currently being in progress within QualityNano Network. The same protocol was used to evaluate cytotoxicity of the reference NM sample in immortalized bronchial epithelial BEAS-2B cell line. As expected, BEAS-2B cells were more susceptible than cancerous A549 cells. Finally, two other methods were employed to investigate concordance among various cytotoxicity assays. Both metabolic activity-based WST-1 assay and LDH membrane integrity assay have been proven to be relevant for studying NM cytotoxicity.
- Nanomaterials (NMs) have become crucial for many applications promising significant improvement in various aspects of quality of life. Indisputable benefits of NMs, however, might be discredited by concerns about potential adverse health effects arising from unique properties related to the nanosize. Safety assessment of NMs is complicated by the fact that conventional methods are insufficient for evaluating their toxicity. Although new methods adapted for NMs are being extensively introduced by toxicologists, the lack of standardization causes inconsistency in existing toxicity data making it impossible to draw general conclusions about NM toxicity. QualityNano is a current European FP7 project aimed at developing and implementing best practice and quality in nanosafety assessment. As one of the participating laboratories, we evaluated cytotoxic potential of two reference NMs (non-cytotoxic 40nm carboxy-functionalized and cytotoxic 50nm amino-functionalized polystyrene nanoparticles) in lung adenocarcinoma A549 cell line using QualityNano Standard Operation Procedure for MTS Cell Viability Assay. Results of two performers showed good intra-laboratory concordance and obtained data were provided for inter-laboratory variation assessment which is currently being in progress within QualityNano Network. The same protocol was used to evaluate cytotoxicity of the reference NM sample in immortalized bronchial epithelial BEAS-2B cell line. As expected, BEAS-2B cells were more susceptible than cancerous A549 cells. Finally, two other methods were employed to investigate concordance among various cytotoxicity assays. Both metabolic activity-based WST-1 assay and LDH membrane integrity assay have been proven to be relevant for studying NM cytotoxicity. (en)
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| Title
| - Cytotoxicity Induced by Nanoparticles – Standardisation of Methods within QualityNano FP7
- Cytotoxicity Induced by Nanoparticles – Standardisation of Methods within QualityNano FP7 (en)
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| skos:prefLabel
| - Cytotoxicity Induced by Nanoparticles – Standardisation of Methods within QualityNano FP7
- Cytotoxicity Induced by Nanoparticles – Standardisation of Methods within QualityNano FP7 (en)
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| skos:notation
| - RIV/61989100:27200/14:86091119!RIV15-MSM-27200___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989100:27200/14:86091119
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Nanomaterials, Cytotoxicity, Standardization, QualityNano (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - NANOCON 2014: 6th International Conference, November 5th-7th 2014, Hotel Voronez I, Brno, Czech Republic, EU
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Danihelka, Pavel
- Nováková, Zuzana
- Topinka, Jan
- Brzicová, Táňa
- Rössner, Pavel, Jr.
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
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