About: A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis

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About: A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Euglena gracilis possesses secondary plastids of green algal origin. In this study, E. gracilis expressed sequence tags (ESTs) derived from polyA-selected mRNA were searched and several ESTs corresponding to plastid genes were found. PCR experiments failed to detect SL sequence at the 5'-end of any of these transcripts, suggesting plastid origin of these polyadenylated molecules. Quantitative PCR experiments confirmed that polyadenylation of transcripts occurs in the Euglena plastids. Such transcripts have been previously observed in primary plastids of plants and algae as low-abundance intermediates of transcript degradation. Our results suggest that a similar mechanism exists in secondary plastids. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. (en)
Title	A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis (en)
skos:prefLabel	A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis A small portion of plastid transcripts is polyadenylated in the flagellate Euglena gracilis (en)
skos:notation	RIV/61988987:17310/14:A1501CH4!RIV15-MSM-17310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(EE2.3.30.0047), P(GA13-24983S)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Vesteg, Matej Záhonová, Kristína Eliáš, Marek Yurchenko, Vyacheslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ostravská univerzita v Ostravě / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	1188
http://linked.open...ai/riv/idVysledku	RIV/61988987:17310/14:A1501CH4
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Plastid; EST; Euglenozoa; Polyadenylation; Quantitative PCR; Trans-splicing (en)
http://linked.open.../riv/klicoveSlovo	Euglenozoa Polyadenylation Quantitative PCR EST Trans-splicing Plastid
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C931D1678EC8]
http://linked.open...i/riv/nazevZdroje	FEBS LETTERS
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Unravelling the early evolution of the eukaryotic cell through exploring the genomes of the eukaryotic superphylum Discoba Strengthening research institutions at the University of Ostrava
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	588
http://linked.open...iv/tvurceVysledku	Eliáš, Marek Vesteg, Matej Yurchenko, Vyacheslav Hadariová, L. Krajčovič, J. Vacula, R. Záhonová, Kristína
http://linked.open...ain/vavai/riv/wos	000331717500022
issn	0014-5793
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	17310

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