About: Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes

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About: Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress. Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress. (en)
Title	Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes (en)
skos:prefLabel	Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes (en)
skos:notation	RIV/61389030:_____/14:00440405!RIV15-GA0-61389030
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(ED0007/01/01), P(ED2.1.00/03.0058), P(GA522/09/0083), P(GAP501/12/2220), P(GBP501/12/G090), P(GP13-34962P), S
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Šafář, Jan Doležel, Jaroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	16167
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/14:00440405
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Copper; Gene duplication; Metallothionein (en)
http://linked.open.../riv/klicoveSlovo	Copper Metallothionein Gene duplication
http://linked.open...odStatuVydavatele	AT - Rakouská republika
http://linked.open...ontrolniKodProRIV	[4DC280C25C15]
http://linked.open...i/riv/nazevZdroje	Protoplasma
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Regional Centre of Advanced Technologies and Materials Sex chromosome evolution - chromosome-specific genomics in genus Silene Centre of the Region Haná for Biotechnological and Agricultural Research Evolution and Function of Complex Plant Genomes Isolation of Sex Linked Genes to Study Evolution of Sex Chromosomes in Plants Silene dioica as a model species for heavy metal tolerance
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	251
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Šafář, Jan Vyskot, Boris Hobza, Roman Čegan, Radim Baloun, Jiří Hudzieczek, Vojtěch Nevrtalová, Eva Milde, D.
http://linked.open...ain/vavai/riv/wos	000344336500013
issn	0033-183X
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00709-014-0644-x

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