About: Proteomic analysis of barley cell nuclei purified by flow sorting

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25059295
Description	Many proteins are present in the nucleus; some are involved with its structural and functional organization, some with gene expression, and some with cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both the artifactual alteration of the proteins and the extent of contamination with non-nuclear proteins. The conventional multi-step fractionation procedure is both laborious and prone to contamination. Here, we describe a single-step method based on flow sorting. The method allows the separation of G1, S and G2 phase nuclei and minimizes the risk of contamination by non-nuclear proteins. Preliminary results obtained using G1 phase cell nuclei from barley root tips indicate that flow sorting coupled with a protein/peptide separation and mass spectrometry will permit a comprehensive characterization of the plant nuclear proteome. Many proteins are present in the nucleus; some are involved with its structural and functional organization, some with gene expression, and some with cell division. The plant nuclear proteome has not been well explored. Its characterization requires extraction methods which minimize both the artifactual alteration of the proteins and the extent of contamination with non-nuclear proteins. The conventional multi-step fractionation procedure is both laborious and prone to contamination. Here, we describe a single-step method based on flow sorting. The method allows the separation of G1, S and G2 phase nuclei and minimizes the risk of contamination by non-nuclear proteins. Preliminary results obtained using G1 phase cell nuclei from barley root tips indicate that flow sorting coupled with a protein/peptide separation and mass spectrometry will permit a comprehensive characterization of the plant nuclear proteome. (en)
Title	Proteomic analysis of barley cell nuclei purified by flow sorting Proteomic analysis of barley cell nuclei purified by flow sorting (en)
skos:prefLabel	Proteomic analysis of barley cell nuclei purified by flow sorting Proteomic analysis of barley cell nuclei purified by flow sorting (en)
skos:notation	RIV/61389030:_____/14:00433901!RIV15-GA0-61389030
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(EE2.3.20.0165), P(GA14-28443S), P(GBP501/12/G090), P(LO1204), S
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Petrovská, Beáta Doležel, Jaroslav Vrána, Jan Jeřábková, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	40483
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/14:00433901
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cell cycle; Chromatin; Flow cytometry (en)
http://linked.open.../riv/klicoveSlovo	Cell cycle Flow cytometry Chromatin
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[C13B0676931B]
http://linked.open...i/riv/nazevZdroje	Cytogenetics and Genome Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Integration of Research Team of the Centre of the Region Haná into International Collaboration Udržitelný rozvoj výzkumu v Centru regionu Haná Evolution and Function of Complex Plant Genomes Dark matter in plant cell nuclei – characterization of nuclear proteins
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	143
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Petrovská, Beáta Šebela, M. Vrána, Jan Jeřábková, Hana Chamrád, I. Lenobel, R. Uřinovská, J.
http://linked.open...ain/vavai/riv/wos	000342614100008
issn	1424-8581
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1159/000365311

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