About: The use of FM dyes to analyze plant endocytosis

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About: The use of FM dyes to analyze plant endocytosis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25117271
Description	FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes. Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery. FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes. Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery. (en)
Title	The use of FM dyes to analyze plant endocytosis The use of FM dyes to analyze plant endocytosis (en)
skos:prefLabel	The use of FM dyes to analyze plant endocytosis The use of FM dyes to analyze plant endocytosis (en)
skos:notation	RIV/61389030:_____/14:00433745!RIV15-GA0-61389030
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP305/11/2476), P(GPP305/11/P797)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Petrášek, Jan Černá, Adriana Malínská, Kateřina
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	52284
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/14:00433745
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Endocytosis; Endomembranes; FM 1-43 (en)
http://linked.open.../riv/klicoveSlovo	Endocytosis FM 1-43 Endomembranes
http://linked.open...ontrolniKodProRIV	[F63E224340BF]
http://linked.open...i/riv/mistoVydani	Totowa
http://linked.open...vEdiceCisloSvazku	Methods in Molecular Biology
http://linked.open...i/riv/nazevZdroje	Plant Endosomes - Methods and Protocols
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	283 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Transport auxinu a cytoskelet v morfogenezi rostlinných buněk. Cytoskeletal proteins mediating targeted deposition of auxin transporters
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Petrášek, Jan Malínská, Kateřina Jelínková, Adriana
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-4939-1420-3_1
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-4939-1419-7

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