About: Determination of Auxin Transport Parameters on the Cellular Level

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin. The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe the method for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the activity of those carriers. The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin. The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe the method for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the activity of those carriers. (en)
Title	Determination of Auxin Transport Parameters on the Cellular Level Determination of Auxin Transport Parameters on the Cellular Level (en)
skos:prefLabel	Determination of Auxin Transport Parameters on the Cellular Level Determination of Auxin Transport Parameters on the Cellular Level (en)
skos:notation	RIV/61389030:_____/14:00429994!RIV15-AV0-61389030
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Zažímalová, Eva Petrášek, Jan Laňková, Martina
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	10737
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/14:00429994
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Auxin transport assay; Plant cell cultures; Auxin influx (en)
http://linked.open.../riv/klicoveSlovo	Auxin influx Auxin transport assay Plant cell cultures
http://linked.open...ontrolniKodProRIV	[F63B7C77990D]
http://linked.open...i/riv/mistoVydani	Totowa
http://linked.open...i/riv/nazevZdroje	Plant Chemical Genomics
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...v/pocetStranKnihy	269 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Petrášek, Jan Zažímalová, Eva Laňková, Martina
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-592-7_22
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-62703-591-0

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