About: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

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About: Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The moss Physcomitrella patens is unique for the high frequency of homologous recombination, haploid state, and filamentous growth during early stages of the vegetative growth, which makes it an excellent model plant to study DNA damage responses. We used single cell gel electrophoresis (comet) assay to determine kinetics of response to Bleomycin induced DNA oxidative damage and single and double strand breaks in wild type and mutant lig4 Physcomitrella lines. Moreover, APT gene when inactivated by induced mutations was used as selectable marker to ascertain mutational background at nucleotide level by sequencing of the APT locus. We show that extensive repair of DSBs occurs also in the absence of the functional LIG4, whereas repair of SSBs is seriously compromised. From analysis of induced mutations we conclude that their accumulation rather than remaining lesions in DNA and blocking progression through cell cycle is incompatible with normal plant growth and development and leads to sensitive phenotype. The moss Physcomitrella patens is unique for the high frequency of homologous recombination, haploid state, and filamentous growth during early stages of the vegetative growth, which makes it an excellent model plant to study DNA damage responses. We used single cell gel electrophoresis (comet) assay to determine kinetics of response to Bleomycin induced DNA oxidative damage and single and double strand breaks in wild type and mutant lig4 Physcomitrella lines. Moreover, APT gene when inactivated by induced mutations was used as selectable marker to ascertain mutational background at nucleotide level by sequencing of the APT locus. We show that extensive repair of DSBs occurs also in the absence of the functional LIG4, whereas repair of SSBs is seriously compromised. From analysis of induced mutations we conclude that their accumulation rather than remaining lesions in DNA and blocking progression through cell cycle is incompatible with normal plant growth and development and leads to sensitive phenotype. (en)
Title	Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens (en)
skos:prefLabel	Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens (en)
skos:notation	RIV/61389030:_____/13:00423951!RIV14-MSM-61389030
http://linked.open...avai/predkladatel	Ústav experimentální botaniky AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(LD13006)
http://linked.open...iv/cisloPeriodika	ID 535049
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Vágnerová, Radka Angelis, Karel Holá, Marcela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	76429
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/13:00423951
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA-DAMAGE; COMET ASSAY; ARABIDOPSIS-THALIANA (en)
http://linked.open.../riv/klicoveSlovo	COMET ASSAY ARABIDOPSIS-THALIANA DNA-DAMAGE
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[85936CF0F53F]
http://linked.open...i/riv/nazevZdroje	BIOMED RESEARCH INTERNATIONAL
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular basis of plant response to UV-B radiation
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Angelis, Karel Kozák, Jaroslav Holá, Marcela Vágnerová, Radka
http://linked.open...ain/vavai/riv/wos	000328834200001
issn	2314-6133
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1155/2013/535049

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