About: Flow cytometric chromosome sorting in plants: The next generation

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing. Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing. (en)
Title	Flow cytometric chromosome sorting in plants: The next generation Flow cytometric chromosome sorting in plants: The next generation (en)
skos:prefLabel	Flow cytometric chromosome sorting in plants: The next generation Flow cytometric chromosome sorting in plants: The next generation (en)
skos:notation	RIV/61389030:_____/12:00381153!RIV13-AV0-61389030
http://linked.open...avai/predkladatel	Ústav experimentální botaniky AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP501/10/1740), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Doležel, Jaroslav Šimková, Hana Kubaláková, Marie Číhalíková, Jarmila Vrána, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	136827
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/12:00381153
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chromosome sorting; Flow cytometry; Fluorescence in situ hybridization (en)
http://linked.open.../riv/klicoveSlovo	Chromosome sorting Flow cytometry Fluorescence in situ hybridization
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2DA843F49310]
http://linked.open...i/riv/nazevZdroje	Methods
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Physical map of the wheat chromosome 4AL and positional cloning of a gene for yield
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	57
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Kubaláková, Marie Číhalíková, Jarmila Šimková, Hana Vrána, Jan
http://linked.open...ain/vavai/riv/wos	000308852400009
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1046-2023
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ymeth.2012.03.006
is http://linked.open...avai/riv/vysledek of	Flow cytometric chromosome sorting in plants: The next generation

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