About: The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes the viral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3'-terminal modification of XCP on the chimera's life cycle. To ensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3'-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP was mutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras. The Potato virus X (PVX)-based vector was used for the construction of N- and C-terminally modified PVX coat protein (XCP) chimeras. N-terminal XCP modifications do not influence the viral life cycle, whereas the simple XCP C-terminal fusion impedes the viral replication. We designed several C-terminally modified XCP chimeras and tested their viabilities in various Nicotiana benthamiana genotypes. Our results showed the negative impact of 3'-terminal modification of XCP on the chimera's life cycle. To ensure chimeric constructs stability, the second copy of the last 60 nucleotides of XCP followed by the 3'-untranslated region (UTR) was added downstream of the recombinant sequence. Simultaneously, the first copy of the last 60 nucleotides of XCP was mutated in order to prevent recombination between the two identical sequences. The movement protein of Tobacco mosaic virus expressed in transgenic N. benthamiana plants positively affected the cell-to-cell spread of C-terminally modified XCP chimeras. (en)
Title	The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties (en)
skos:prefLabel	The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties (en)
skos:notation	RIV/61389030:_____/12:00381055!RIV13-AV0-61389030
http://linked.open...avai/predkladatel	Ústav experimentální botaniky AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GA521/09/1525), S, Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Čeřovská, Noemi Folwarczna, Jitka Moravec, Tomáš Hoffmeisterová, Hana Plchová, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	141633
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/12:00381055
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	chimeric coat protein; expression of recombinant protein; Nicotiana benthamiana (en)
http://linked.open.../riv/klicoveSlovo	Nicotiana benthamiana chimeric coat protein expression of recombinant protein
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[2508F1212D89]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Biosafe plant virus expression system for transient production of human papillomavirus oncoproteins and their use for therapeutic vaccine development
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Folwarczna, Jitka Hoffmeisterová, Hana Plchová, Helena Čeřovská, Noemi
http://linked.open...ain/vavai/riv/wos	000305602100027
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0006-3134
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10535-012-0130-2
is http://linked.open...avai/riv/vysledek of	The influence of the N- and C- terminal modifications of Potato virus X coat protein on virus properties

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