About: Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFPKDEL reporter protein upon addition of the chemical ligand, methoxyfenozide. The chemical gene switch activated gene expression under in vitro conditions in somatic cotyledonary embryos and zygotic seed embryos cultured from transgenic soybean plants, as well as in seeds in planta under greenhouse conditions. The efficiency of induction of GFP expression under different growth conditions was strongly influenced by the developmental stage of the seed and availability of the inducer. The formation of ER-derived GFP-containing protein bodies in seed storage parenchyma cells was correlated with the level of induced expression. Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFPKDEL reporter protein upon addition of the chemical ligand, methoxyfenozide. The chemical gene switch activated gene expression under in vitro conditions in somatic cotyledonary embryos and zygotic seed embryos cultured from transgenic soybean plants, as well as in seeds in planta under greenhouse conditions. The efficiency of induction of GFP expression under different growth conditions was strongly influenced by the developmental stage of the seed and availability of the inducer. The formation of ER-derived GFP-containing protein bodies in seed storage parenchyma cells was correlated with the level of induced expression. (en)
Title	Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds (en)
skos:prefLabel	Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds (en)
skos:notation	RIV/61389030:_____/10:00348486!RIV11-AV0-61389030
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Moravec, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	245352
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00348486
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Inducible expression; Soybean; Seed-specific genes (en)
http://linked.open.../riv/klicoveSlovo	Soybean Inducible expression Seed-specific genes
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[4962DABF6A38]
http://linked.open...i/riv/nazevZdroje	Transgenic Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Beachy, R. N. Schmidt, M. A. Semenyuk, E. G. Woodford-Thomas, T.
http://linked.open...ain/vavai/riv/wos	000286110200005
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0962-8819
number of pages	13 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 112 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software