About: Probing plant membranes with FM dyes: tracking, dragging or blocking?

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. Remarkable progress in various techniques of in vivo fluorescence microscopy has brought an urgent need for reliable markers for tracking cellular structures and processes. The goal of this manuscript is to describe unexplored effects of the FM (Fei Mao) styryl dyes, which are widely used probes that label processes of endocytosis and vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. (en)
Title	Probing plant membranes with FM dyes: tracking, dragging or blocking? Probing plant membranes with FM dyes: tracking, dragging or blocking? (en)
skos:prefLabel	Probing plant membranes with FM dyes: tracking, dragging or blocking? Probing plant membranes with FM dyes: tracking, dragging or blocking? (en)
skos:notation	RIV/61389030:_____/10:00343962!RIV11-MSM-61389030
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(KJB600380604), P(LC06034), S, Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Zažímalová, Eva Pařezová, Markéta Petrášek, Jan Černá, Adriana Martinec, Jan Pejchar, Přemysl Malínská, Kateřina Simon, Sibu Kubeš, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	282115
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00343962
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	FM 4-64; FM 1-43; plasma membrane (en)
http://linked.open.../riv/klicoveSlovo	plasma membrane FM 1-43 FM 4-64
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[21B5DE275C87]
http://linked.open...i/riv/nazevZdroje	Plant Journal
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	9 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation of morphogenesis of plant cells and organs Mechanisms of intracellular trafficking and polar targeting of auxin efflux facilitators.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	61
http://linked.open...iv/tvurceVysledku	Friml, J. Martinec, Jan Pařezová, Markéta Petrášek, Jan Zažímalová, Eva Kleine-Vehn, J. Pejchar, Přemysl Malínská, Kateřina Simon, Sibu Kubeš, Martin Jelínková, Adriana
http://linked.open...ain/vavai/riv/wos	000274805600014
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0960-7412
number of pages	10 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Probing plant membranes with FM dyes: tracking, dragging or blocking? Probing plant membranes with FM dyes: tracking, dragging or blocking? Probing plant membranes with FM dyes: tracking, dragging or blocking?

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