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Description
| - The cytokinin biosynthesis gene, isopentenyl transferase (ipt), under the control of a 821 bp fragment of the LEACO1 gene promoter (from Lycopersicon esculentum) was introduced into Dendranthema x grandiflorium Iridon’ (chrysanthemum). LEACO10.821kb-ipt transgenic lines exhibited in the vegetative state enhanced branching and reduced internode lengths. In the generative state, transformed lines exhibited elevated flower bud counts (3.8 – 6.7x more than WT), which was associated with a delay of flower bud development and a decrease in flower bud diameter. RT-PCR analysis indicated differences in ipt gene expression between individual transgenic lines, as well as at different stages of generative shoot development. Expression of ipt in transgenic lines correlated well with high concentrations of endogenous cytokinins. In general, transgenic lines accumulated more cytokinins, including cytokinin O-glucosides and N-glucosides in generative shoots than in the vegetative ones.
- The cytokinin biosynthesis gene, isopentenyl transferase (ipt), under the control of a 821 bp fragment of the LEACO1 gene promoter (from Lycopersicon esculentum) was introduced into Dendranthema x grandiflorium Iridon’ (chrysanthemum). LEACO10.821kb-ipt transgenic lines exhibited in the vegetative state enhanced branching and reduced internode lengths. In the generative state, transformed lines exhibited elevated flower bud counts (3.8 – 6.7x more than WT), which was associated with a delay of flower bud development and a decrease in flower bud diameter. RT-PCR analysis indicated differences in ipt gene expression between individual transgenic lines, as well as at different stages of generative shoot development. Expression of ipt in transgenic lines correlated well with high concentrations of endogenous cytokinins. In general, transgenic lines accumulated more cytokinins, including cytokinin O-glucosides and N-glucosides in generative shoots than in the vegetative ones. (en)
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Title
| - Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter
- Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter (en)
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skos:prefLabel
| - Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter
- Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter (en)
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skos:notation
| - RIV/61389030:_____/09:00337485!RIV10-AV0-61389030
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/09:00337485
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - ipt Gene; Cytokinins; Transgenic chrysanthemum (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Malbeck, Jiří
- Vaňková, Radomíra
- Li, Y.
- Khodakovskaya, M.
- Li, A.
- McAvoy, R.
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http://linked.open...ain/vavai/riv/wos
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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