| Attributes | Values |
|---|
| rdf:type
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| Description
| - Exocyst je oktamerický poutací komplex. Skládá se z osmi podjednotek - SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 a EXO84. Prozatím byl charakterizován na molekulární úrovni u kvasinek a živočichů. V této práci přinášíme genetická, molekulárně biologická a biochemická data ukazující, že tento komplex funguje podobným způsobem také v rostlinné buňce, kde se podílí na klíčových dějích polárního růstu (klíčení pylové láčky, etiolovaný hypokotyl). Podařilo se nám také ukázat vzájemné interakce jednotlivých podjednotek, které přinášejí informaci o celkové struktuře komplexu. (cs)
- The exocyst, an octameric tethering complex and effector of Rho and Rab GTPases, facilitates polarized secretion in yeast and animals. Recent evidence implicates three plant homologs of exocyst subunits (SEC3, SEC8, and EXO70A1) in plant cell morphogenesis. Here, we provide genetic, cell biological, and biochemical evidence that these and other predicted subunits function together in vivo in Arabidopsis thaliana. Double mutants in exocyst subunits (sec5 exo70A1 and sec8 exo70A1) show a synergistic defect in etiolated hypocotyl elongation. Mutants in exocyst subunits SEC5, SEC6, SEC8, and SEC15a show defective pollen germination and pollen tube growth phenotypes. Using antibodies directed against SEC6, SEC8, and EXO70A1, we demonstrate colocalization of these proteins at the apex of growing tobacco pollen tubes. The SEC3, SEC5, SEC6, SEC8, SEC10, SEC15a, and EXO70 subunits copurify in a high molecular mass fraction of 900 kD after chromatographic fractionation of an Arabidopsis cell suspension extra...
- The exocyst, an octameric tethering complex and effector of Rho and Rab GTPases, facilitates polarized secretion in yeast and animals. Recent evidence implicates three plant homologs of exocyst subunits (SEC3, SEC8, and EXO70A1) in plant cell morphogenesis. Here, we provide genetic, cell biological, and biochemical evidence that these and other predicted subunits function together in vivo in Arabidopsis thaliana. Double mutants in exocyst subunits (sec5 exo70A1 and sec8 exo70A1) show a synergistic defect in etiolated hypocotyl elongation. Mutants in exocyst subunits SEC5, SEC6, SEC8, and SEC15a show defective pollen germination and pollen tube growth phenotypes. Using antibodies directed against SEC6, SEC8, and EXO70A1, we demonstrate colocalization of these proteins at the apex of growing tobacco pollen tubes. The SEC3, SEC5, SEC6, SEC8, SEC10, SEC15a, and EXO70 subunits copurify in a high molecular mass fraction of 900 kD after chromatographic fractionation of an Arabidopsis cell suspension extra... (en)
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| Title
| - An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco
- An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco (en)
- Komplex Exocyst se účastní na růstu rostlinné buňky v Arabidopsis a tabáku (cs)
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| skos:prefLabel
| - An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco
- An Exocyst Complex Functions in Plant Cell Growth in Arabidopsis and Tobacco (en)
- Komplex Exocyst se účastní na růstu rostlinné buňky v Arabidopsis a tabáku (cs)
|
| skos:notation
| - RIV/61389030:_____/08:00318543!RIV09-AV0-61389030
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(IAA6038410), P(LC06034), P(ME 841), Z(AV0Z50380511), Z(MSM0021620858)
|
| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/08:00318543
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - EXOCYST; PROTEIN COMPLEX; CELL POLARITY; MORPHOGENESIS; GTPASES (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Hála, Michal
- Žárský, Viktor
- Drdová, Edita
- Pečenková, Tamara
- Synek, Lukáš
- Fowler, J. E.
- Lamkemeyer, T.
- Cole, R. A.
- Hochholdinger, F.
- Madlung, J.
- Nordheim, A.
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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