| Attributes | Values |
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| rdf:type
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| Description
| - Byla optimalizována exprese rekombinantního obalového proteinu A viru bramboru (ACP) nesoucího dva různé epitopy z lidského papillomaviru typu 16 (HPV16). Epitop odvozený od capsidového proteinu L2 byl exprímován jako N-terminální fuze obalového proteinu A viru bramboru, kdežto epitop odvozený z E7 oncoproteinu byl fúzován k jeho C-konci. Konstrukt byl klónován do vektoru odvozeného od X viru bramboru a za použití Agrobacteria tumefaciens byl transientně exprimován v rostlinách. Pro zvýšení hladiny exprimovaného proteinu byly testovány transgenní rostliny Nicotina benthamiana exprimující HC-pro protein z A viru bramboru a transgenní Nicotiana tabacum, cv. Petit Havana SR 1 nesoucí P3 protein A viru bramboru. Synergistická infekce hostitelských rostlin vektorem nesoucím konstrukt a Y° virem bramboru (PVY°) zvyšovala expresi L2ACPE7 v N. tabacum a v transgenních N. benthamiana nesoucích potyviral HC-Pro gen ve srovnání s kontrolními rostlinami infikovanými pouze s L2ACPE7. (cs)
- The optimized expression of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The construct was cloned into Potato X potexvirus (PVX) based vector and transiently expressed in plants using Agrobacterium tumefaciens mediated inoculation. To increase the level of expressed protein the transgenic Nicotiana benthamiana plants expressing Potato virus A HC-Pro gene and transgenic Nicotiana tabacum, cv. Petit Havana SR1 carrying Potato virus A P3 protein gene were tested. Synergistic infection of host plants with PVX carrying the construct and Potato virus Y-o (PVYO) increased the expression of L2ACPE7 in N. tabacum and in transgenic N. benthamiana carrying potyviral HC-Pro gene as compared to control plants infected with L2ACPE7 only.
- The optimized expression of recombinant Potato virus A coat protein (ACP) carrying two different epitopes from Human papillomavirus type 16 (HPV16) was developed. Epitope derived from minor capsid protein L2 was expressed as N-terminal fusion with ACP while an epitope derived from E7 oncoprotein was fused to its C-terminus. The construct was cloned into Potato X potexvirus (PVX) based vector and transiently expressed in plants using Agrobacterium tumefaciens mediated inoculation. To increase the level of expressed protein the transgenic Nicotiana benthamiana plants expressing Potato virus A HC-Pro gene and transgenic Nicotiana tabacum, cv. Petit Havana SR1 carrying Potato virus A P3 protein gene were tested. Synergistic infection of host plants with PVX carrying the construct and Potato virus Y-o (PVYO) increased the expression of L2ACPE7 in N. tabacum and in transgenic N. benthamiana carrying potyviral HC-Pro gene as compared to control plants infected with L2ACPE7 only. (en)
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| Title
| - Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector
- Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector (en)
- Transientní exprese peptidů E7 (aa 44-60) a L2 (aa 108-120) z HPV16 na chimérických viru podobných částicích potyvirů pomocí virového vektoru odvozeného od X viru bramboru (cs)
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| skos:prefLabel
| - Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector
- Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector (en)
- Transientní exprese peptidů E7 (aa 44-60) a L2 (aa 108-120) z HPV16 na chimérických viru podobných částicích potyvirů pomocí virového vektoru odvozeného od X viru bramboru (cs)
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| skos:notation
| - RIV/61389030:_____/08:00305884!RIV08-AV0-61389030
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA521/06/0973), V, Z(AV0Z50380511)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/08:00305884
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - epitopes expression; Human papillomavirus; transient expression; potyvirus-like particles; synergistic infection (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Protein Expression and Purification
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Moravec, Tomáš
- Hoffmeisterová, Hana
- Plchová, Helena
- Čeřovská, Noemi
- Pečenková, Tamara
- Synková, Helena
- Velemínský, Jiří
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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