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| Description
| - Aim of the study was to investigate the impact of coffee on DNA-stability in humans. DNA-damage was monitored in lymphocytes of eight individuals with single cell gel electrophoresis assays before and after consumption of 600 ml coffee (400 ml paper filtered and 200 ml metal filtered/d) for five days. Under standard conditions, no alteration of DNA-migration was seen, but a strong reduction of DNA-migration attributable to endogenous formation of oxidised purines and pyrimidines was detected with restriction enzymes; furthermore DNA-damage caused by reactive oxygen radicals (H2O2 treatment) and by the heterocyclic aromatic amine 3-amino-1- methyl-5H-pyrido[4,3-b]indole-acetate was significantly reduced after coffee consumption by 17% and 35%, respectively. Also in in vitro experiments, inhibition of H2O2 induced DNA-damage was observed with coffee at low concentrations (625 ll/ml) whereas the diterpenoids cafestol and kahweol caused only marginal effects indicating that the effects of coffee are d...
- Aim of the study was to investigate the impact of coffee on DNA-stability in humans. DNA-damage was monitored in lymphocytes of eight individuals with single cell gel electrophoresis assays before and after consumption of 600 ml coffee (400 ml paper filtered and 200 ml metal filtered/d) for five days. Under standard conditions, no alteration of DNA-migration was seen, but a strong reduction of DNA-migration attributable to endogenous formation of oxidised purines and pyrimidines was detected with restriction enzymes; furthermore DNA-damage caused by reactive oxygen radicals (H2O2 treatment) and by the heterocyclic aromatic amine 3-amino-1- methyl-5H-pyrido[4,3-b]indole-acetate was significantly reduced after coffee consumption by 17% and 35%, respectively. Also in in vitro experiments, inhibition of H2O2 induced DNA-damage was observed with coffee at low concentrations (625 ll/ml) whereas the diterpenoids cafestol and kahweol caused only marginal effects indicating that the effects of coffee are d... (en)
- Cílem studie bylo zjistit vliv pití kávy na stabilitu DNA u lidí. Poškození DNA bylo analyzováno po požití 0,6 lt kávy u 8 jedinců po dobu 5ti dnů. Přímý vliv kávy nebyl pozorován, ale po indukci poškození DNA kyslíkovými radikály pomocí H2O2 a heterocyklickým a 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetatem (Trp-P-2) bylo zjištěno snížení poškození o 17, respektive 35%. Měřením enzymové aktivity byla zjištěna indukce antioxydačních enzymů jako superoxiddismutasy. Porovnáním s výsledky předchozích studií ukázalo, že pití kávy je účinější prevence proti oxidativnímu poškození DNA než strava bohatá na ovoce a zeleninu. (cs)
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| Title
| - Coffee consumption protects human lymphocytes against oxidative and 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) induced DNA-damage: Results of an experimental study with human volunteers
- Konzumace kávy chrání lidské lymfocyty proti oxidativnímu a 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) indukovanému poškození DNA: studie s dobrovolníky (cs)
- Coffee consumption protects human lymphocytes against oxidative and 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) induced DNA-damage: Results of an experimental study with human volunteers (en)
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| skos:prefLabel
| - Coffee consumption protects human lymphocytes against oxidative and 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) induced DNA-damage: Results of an experimental study with human volunteers
- Konzumace kávy chrání lidské lymfocyty proti oxidativnímu a 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) indukovanému poškození DNA: studie s dobrovolníky (cs)
- Coffee consumption protects human lymphocytes against oxidative and 3-amino-1-methyl-5H-pyrido[4,3-b]indole acetate (Trp-P-2) induced DNA-damage: Results of an experimental study with human volunteers (en)
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| skos:notation
| - RIV/61389030:_____/07:00099631!RIV08-AV0-61389030
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/07:00099631
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - coffee; comet assay; DNA-damage (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Food and Chemical Toxicology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Angelis, Karel
- Kundi, M.
- Chakraborty, A.
- Bichler, J.
- Ferk, F.
- Haidinger, G.
- Hoelzl, C.
- Knasmüller, S.
- Simic, T.
- Cavin, C.
- Schulte-Hermann, R.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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