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| Description
| - Pro poznání úlohy mitochondriální farnesyl difosfát syntázy (FPS) v biosyntéze isoprenoidů jsme použili rostlin Arabidospsis se zvýšenou expresí její mitochondriální isoformy (FPS1L). Bylo potvrzeno, že příslušná aktivní 40 kDa bílkovina je cílená do mitochondrií. Transgenní rostliny pěstované za nepřetržitého osvětlení vykazovaly symptomy chlorózy a buněčné smrti, které korelovaly s produkcí H2O2. V oddělených listech byla urychlena jejich senescence. Byl rovněž změněn profil cytokininů – byla snížena akumulace fyziologicky aktivních cytokininů typu trans-zeatinu a isopentenyladeninu. Současně zvýšena exprese reduktázy 3-hydroxy-3-metylglutaryl koenzymu A sice nezabránila chloróze, ale zpomalila senescenci a vrátila hladiny cytokininů na úroveň netransformovaných rostlin. Domníváme se, že zvýšená exprese FPS1L vede k zvýšené spotřebě isopentenyl difosfátu v mitochondriích a touto cestou mění homeostázi cytokininů pro jejichž biosyntézu je využíván společný prekursor. (cs)
- To investigate the role of mitochondrial farnesyl diphosphate synthase (FPS) in plant isoprenoid biosynthesis we characterized transgenic Arabidopsis thaliana plants overexpressing FPS1L isoform. This overexpressed protein was properly targeted to mitochondria yielding a mature and active form of the enzyme of 40 kDa. Leaves from transgenic plants grown under continuous light exhibited symptoms of chlorosis and cell death correlating to H2O2 accumulation, and leaves detached from the same plants displayed accelerated senescence. Overexpression of FPS in mitochondria also led to altered leaf cytokinin profile, with a reduction in the contents of physiologically active trans-zeatin- and isopentenyladenine-type cytokinins and their corresponding riboside monophosphates as well as enhanced levels of cis-zeatin 7-glucoside and storage cytokinin O-glucosides. Overexpression of 3-hydroxy-3-methylglutaryl coenzyme A reductase did not prevent chlorosis in plants overexpressing FPS1L, but did rescue accelera...
- To investigate the role of mitochondrial farnesyl diphosphate synthase (FPS) in plant isoprenoid biosynthesis we characterized transgenic Arabidopsis thaliana plants overexpressing FPS1L isoform. This overexpressed protein was properly targeted to mitochondria yielding a mature and active form of the enzyme of 40 kDa. Leaves from transgenic plants grown under continuous light exhibited symptoms of chlorosis and cell death correlating to H2O2 accumulation, and leaves detached from the same plants displayed accelerated senescence. Overexpression of FPS in mitochondria also led to altered leaf cytokinin profile, with a reduction in the contents of physiologically active trans-zeatin- and isopentenyladenine-type cytokinins and their corresponding riboside monophosphates as well as enhanced levels of cis-zeatin 7-glucoside and storage cytokinin O-glucosides. Overexpression of 3-hydroxy-3-methylglutaryl coenzyme A reductase did not prevent chlorosis in plants overexpressing FPS1L, but did rescue accelera... (en)
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| Title
| - Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis
- Zvýšená exprese farnesyl difosfát syntázy vyvolává na světle tvorbu lézí a pozměňuje homeostázi cytokininů (cs)
- Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis (en)
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| skos:prefLabel
| - Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis
- Zvýšená exprese farnesyl difosfát syntázy vyvolává na světle tvorbu lézí a pozměňuje homeostázi cytokininů (cs)
- Overexpression of Farnesyl Diphosphate Synthase in Arabidopsis Mitochondria Triggers Light-dependent Lesion Formation and Alters Cytokinin Homeostasis (en)
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| skos:notation
| - RIV/61389030:_____/06:00082318!RIV07-AV0-61389030
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(IAA600380507), Z(AV0Z50380511)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/06:00082318
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Arabidopsis thaliana; cytokinin; farnesyl diphosphate synthase; isoprenoid (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Ferrer, A.
- Hoyerová, Klára
- Kamínek, Miroslav
- Arró, M.
- Busquets, A.
- Closa, M.
- Manzano, D.
- Schaller, H.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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