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| Description
| - Cytokinins are a class of phytohormones that play a critical role in plant growth and development. sob5-D, an activation-tagging mutant, shows phenotypes typical of transgenic plants expressing the Agrobacterium tumefaciens isopentenyltransferase (ipt) gene that encodes the enzyme catalyzing the first step of cytokinin biosynthesis. The sob5-D mutant phenotypes are caused by over-expression of a novel gene, SOB5. Sequence analysis places SOB5 in a previously uncharacterized family of plant-specific proteins. A translational fusion between SOB5 and the green fluorescent protein reporter was localized in the cytoplasm as well as associated with the plasma membrane when transiently expressed in onion epidermal cells. Analysis of transgenic plants harboring an SOB5:SOB5–β-glucuronidase (GUS) translational fusion under the control of the SOB5 promoter region showed GUS activity in vegetative tissues (hydathodes and trichomes of leaves, shoot meristems and roots) as well as in floral tissues (pistil tips...
- Cytokinins are a class of phytohormones that play a critical role in plant growth and development. sob5-D, an activation-tagging mutant, shows phenotypes typical of transgenic plants expressing the Agrobacterium tumefaciens isopentenyltransferase (ipt) gene that encodes the enzyme catalyzing the first step of cytokinin biosynthesis. The sob5-D mutant phenotypes are caused by over-expression of a novel gene, SOB5. Sequence analysis places SOB5 in a previously uncharacterized family of plant-specific proteins. A translational fusion between SOB5 and the green fluorescent protein reporter was localized in the cytoplasm as well as associated with the plasma membrane when transiently expressed in onion epidermal cells. Analysis of transgenic plants harboring an SOB5:SOB5–β-glucuronidase (GUS) translational fusion under the control of the SOB5 promoter region showed GUS activity in vegetative tissues (hydathodes and trichomes of leaves, shoot meristems and roots) as well as in floral tissues (pistil tips... (en)
- Cytokininy jsou fytohormony, které mají rozhodující roli při regulaci růstu a vývoje rostlin. Mutant sob5-D vykazuje fenotyp typický pro transgenní rostliny exprimující gen z Agrobacteria tumefaciens kódující isopentenyltransferázu (ipt), enzym, který katalyzuje první krok biosyntézy cytokininů. V mutantu sob5 je over-exprimován gen SOB5, kódující rostlinný protein z dosud necharakterizované rodiny. Po translační fúzi s genem pro zelený fluorescenční protein (GFP), byla exprese SOB5 lokalizována v cytoplasmě a v plazmatické membráně (při použití epidermálních buněk cibule). Analýza rostlin Arabidopsis, které mají konstrukt SOB5 s genem pro GUS pod přirozeným SOB5 promotorem, prokázala aktivitu GUS jak ve vegetativních tkáních (hydathody a trichomy listů, vrcholové meristémy a kořeny), tak v květních tkáních (blizny, vyvíjející se prašníky, cévní systém kališních plátků). Analýza endogenních cytokininů ukázala zvýšení hladin ribosidu a ribotidu trans-zeatinu a 9-glukosidu isopentenyl adeninu ve srov... (cs)
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| Title
| - Over-expression of SOB5 suggests the involvement of a novel plant protein in cytokinin-mediated development
- Analýza transgenu se zvýšenou expresí SOB5 naznačuje, že se tento nový protein podílí na regulaci vývoje cytokininy (cs)
- Over-expression of SOB5 suggests the involvement of a novel plant protein in cytokinin-mediated development (en)
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| skos:prefLabel
| - Over-expression of SOB5 suggests the involvement of a novel plant protein in cytokinin-mediated development
- Analýza transgenu se zvýšenou expresí SOB5 naznačuje, že se tento nový protein podílí na regulaci vývoje cytokininy (cs)
- Over-expression of SOB5 suggests the involvement of a novel plant protein in cytokinin-mediated development (en)
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| skos:notation
| - RIV/61389030:_____/06:00049310!RIV07-AV0-61389030
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA522/04/0549), Z(AV0Z50380511)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/06:00049310
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - SOB5; SOFL; cytokinin (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Malbeck, Jiří
- Vaňková, Radomíra
- Zhang, J.
- Neff, M. M.
- Wrage, E. L.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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