About: Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors. (cs) The catalytic reaction of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) was studied in detail using the recombinant flavoenzyme from maize. Determination of the redox potential of the covalently linked flavin cofactor revealed a relatively high potential dictating the type of electron acceptor that can be used by the enzyme. Using 2,6-dichlorophenol indophenol, 2,3-dimethoxy-5methyl-1,4-benzoquinone or 1,4-naphthoquinone as electron acceptor, turnover rates with N-6-(2-isopentenyl)adenine of approx. 150 s(-1) could be obtained. This suggests that the natural electron acceptor of the enzyme is quite probably a p-quinone or similar compound. By using the stopped-flow technique, it was found that the enzyme is rapidly reduced by N-6-(2-isopentenyl) adenine (k(red) = 950 s(-1)). Re-oxidation of the reduced enzyme by molecular oxygen is too slow to be of physiological relevance, confirming its classification as a dehydrogenase. Furthermore, it was established for the first time that the... The catalytic reaction of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) was studied in detail using the recombinant flavoenzyme from maize. Determination of the redox potential of the covalently linked flavin cofactor revealed a relatively high potential dictating the type of electron acceptor that can be used by the enzyme. Using 2,6-dichlorophenol indophenol, 2,3-dimethoxy-5methyl-1,4-benzoquinone or 1,4-naphthoquinone as electron acceptor, turnover rates with N-6-(2-isopentenyl)adenine of approx. 150 s(-1) could be obtained. This suggests that the natural electron acceptor of the enzyme is quite probably a p-quinone or similar compound. By using the stopped-flow technique, it was found that the enzyme is rapidly reduced by N-6-(2-isopentenyl) adenine (k(red) = 950 s(-1)). Re-oxidation of the reduced enzyme by molecular oxygen is too slow to be of physiological relevance, confirming its classification as a dehydrogenase. Furthermore, it was established for the first time that the... (en)
Title	Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors (en) Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors (cs)
skos:prefLabel	Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors (en) Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors (cs)
skos:notation	RIV/61389030:_____/04:00109165!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany	121;130
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/03/0979), P(ME 515), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Novák, Ondřej Frébortová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	556912
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/04:00109165
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytokinin dehydrogenase (cytokinin oxidase); cytokinin; flavoprotein (en)
http://linked.open.../riv/klicoveSlovo	cytokinin flavoprotein cytokinin dehydrogenase (cytokinin oxidase)
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FB850D77AE72]
http://linked.open...i/riv/nazevZdroje	Biochemical Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20515
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	380
http://linked.open...iv/tvurceVysledku	Novák, Ondřej Šebela, M. Frébortová, Jitka Galuszka, P. Hrbáč, J. Bilyeu, K. D. Frébort, I. Peč, P. English, J. T. Fraaije, M. W.
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0264-6021
number of pages	10 (xsd:int)

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