About: Transient expression of heterologous model gene in plants using Potato virusX-based vector

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Transient expression of heterologous model gene in plants using Potato virusX-based vector. (cs) To optimize the efficiency of expression of foreign proteins using Potato virus X (PVX) -- based vector, the gene for the coat protein (CP) of other virus (Potato virusA, PVA) was cloned into the vector, propagated in E. coli and subsequently inoculated or agroinfected into the host plants. Host range studies showed that the best host plant is N. benthamiana. By means of RT PCR the presence and the stability of the construct were tested. Both ELISA and Western blot analysis were applicable for expressed protein detection. Expression level of PVA CP achieved approximately 5--10 per mille of total soluble proteins. The results demonstrated that agroinfection is the most suitable method for the propagation of our model gene using PVX--based vectors. To optimize the efficiency of expression of foreign proteins using Potato virus X (PVX) -- based vector, the gene for the coat protein (CP) of other virus (Potato virusA, PVA) was cloned into the vector, propagated in E. coli and subsequently inoculated or agroinfected into the host plants. Host range studies showed that the best host plant is N. benthamiana. By means of RT PCR the presence and the stability of the construct were tested. Both ELISA and Western blot analysis were applicable for expressed protein detection. Expression level of PVA CP achieved approximately 5--10 per mille of total soluble proteins. The results demonstrated that agroinfection is the most suitable method for the propagation of our model gene using PVX--based vectors. (en)
Title	Transient expression of heterologous model gene in plants using Potato virusX-based vector Transient expression of heterologous model gene in plants using Potato virusX-based vector (en) Transient expression of heterologous model gene in plants using Potato virusX-based vector (cs)
skos:prefLabel	Transient expression of heterologous model gene in plants using Potato virusX-based vector Transient expression of heterologous model gene in plants using Potato virusX-based vector (en) Transient expression of heterologous model gene in plants using Potato virusX-based vector (cs)
skos:notation	RIV/61389030:_____/04:00109162!RIV/2005/AV0/A56005/N
http://linked.open.../vavai/riv/strany	147;152
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/00/0381), Z(AV0Z5038910)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Velemínský, Jiří Čeřovská, Noemi Moravec, Tomáš Pečenková, Tamara
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	590643
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/04:00109162
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	plant virus; based vector; transient expression (en)
http://linked.open.../riv/klicoveSlovo	transient expression plant virus based vector
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[7B32D268C7CC]
http://linked.open...i/riv/nazevZdroje	Plant Cell, Tissue and Organ Culture
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Production of human papillomavirus proteins in transgenic plants: an attempt to develop edible vaccines
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Čeřovská, Noemi Pečenková, Tamara Velemínský, Jiří
http://linked.open...n/vavai/riv/zamer	Physiological and genetical bases of plant development, cell cycle, morphogenesis, stress reactions and biotechnologies. Genome organisation and function
issn	0167-6857
number of pages	6 (xsd:int)

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